Microbial rRNA sequencing analysis of evaporative cooler indoor environments located in the Great Basin Desert region of the United States

Details

• Personal Author:
  Gault RA ; Green, Brett J. ; Gu JK ; Hogan MB ; Holland K ; Kashon ML ; Lemons, Angela R. ; Olsen-Wilson KA ; Park Y ; Park, Ju-Hyeong ; Sobek E
• Description:
  Recent studies conducted in the Great Basin Desert region of the United States have shown that skin test reactivity to fungal and dust mite allergens are increased in children with asthma or allergy living in homes with evaporative coolers (EC). The objective of this study was to determine if the increased humidity previously reported in EC homes leads to varying microbial populations compared to homes with air conditioners (AC). Children with physician-diagnosed allergic rhinitis living in EC or AC environments were recruited into the study. Air samples were collected from the child's bedroom for genomic DNA extraction and metagenomic analysis of bacteria and fungi using the Illumina MiSeq sequencing platform. The analysis of bacterial populations revealed no major differences between EC and AC sampling environments. The fungal populations observed in EC homes differed from AC homes. The most prevalent species discovered in AC environments belonged to the genera Cryptococcus (20%) and Aspergillus (20%). In contrast, the most common fungi identified in EC homes belonged to the order Pleosporales and included Alternaria alternata (32%) and Phoma spp. (22%). The variations in fungal populations provide preliminary evidence of the microbial burden children may be exposed to within EC environments in this region. [Description provided by NIOSH]
• Subjects:
  Asthma Bacteria Dust Environmental Monitoring Fungi Humidity Hypersensitivity Microbiology Occupational Health Respiratory System Respiratory Tract Diseases Safety Skin

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• Keywords:
  Air Samples Allergens Aspergillus Infection Bacterial Dusts Bronchial Asthma Children Deoxyribonucleic Acids DNA Dusts Humans Microorganisms Skin Tests

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• ISSN:
  2050-7887
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Nevada ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 5 ; OSHA Region 9 ; Tennessee ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  101-110
• Volume:
  19
• Issue:
  2
• NIOSHTIC Number:
  nn:20049414
• Citation:
  Environ Sci Process Impacts 2017 Feb; 19(2):101-110
• Contact Point Address:
  Angela R. Lemons, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  wrw0@cdc.gov
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Environmental Science: Processes & Impacts
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a343cbd32b3c066f9e2090ee7d1d43fd7a24f3e5d912a369d7893ec206262c1144dce064db5f1b94968105f4ae7f6939e9e3eaf199963efdd2a5a3fc07405c44
• Download URL:
  https://stacks.cdc.gov/view/cdc/203863/cdc_203863_DS1.pdf
• File Type:
  [PDF - 1.48 MB ]