Validation of biomarkers of CVD risk from dried blood spots in community-based research: methodologies and study-specific serum equivalencies

Details

• Personal Author:
  Berkman L ; Buxton OM ; Hall MH ; Marino M ; McDade TW ; McLean S ; Porter JH ; Samuelsson LB ; Sembajwe G
• Description:
  Dried blood spot (DBS) methodology offers significant advantages over venipuncture in studies of vulnerable populations or large-scale studies, including reduced participant burden and higher response rates. Uncertainty about the validity of cardiovascular disease (CVD) risk biomarkers remains a barrier to wide-scale use. We determined the validity of DBS-derived biomarkers of CVD risk versus gold-standard assessments, and study-specific, serum-equivalency values for clinical relevance of DBS-derived values. Concurrent venipuncture serum and DBS samples (n = 150 adults) were assayed in Clinical Laboratory Improvement Amendments-certified and DBS laboratories, respectively. Time controls of DBS standard samples were assayed single-blind along with test samples. Linear regression analyses evaluated DBS-to-serum equivalency values; agreement and bias were assessed via Bland-Altman plots. Linear regressions of venipuncture values on DBS-to-serum equivalencies provided R(2) values for total cholesterol, high-density lipoprotein cholesterol (HDL-C), and C-reactive protein (CRP) of 0.484, 0.118, and 0.666, respectively. Bland-Altman plots revealed minimal systematic bias between DBS-to-serum and venipuncture values; precision worsened at higher mean values of CRP. Time controls revealed little degradation or change in analyte values for HDL-C and CRP over 30 weeks. We concluded that DBS-assessed biomarkers represent a valid alternative to venipuncture assessments. Large studies using DBS should include study-specific serum-equivalency determinations to optimize individual-level sensitivity, the viability of detecting intervention effects, and generalizability in community-level primary prevention interventions. [Description provided by NIOSH]
• Subjects:
  Biological Assay Biomarkers Blood Cardiovascular Diseases Cardiovascular System Hematologic Tests Laboratories Occupational Health Risk Assessment Risk Factors Safety

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• Keywords:
  Analytical Processes Bioassays Blood Analysis Blood Samples Blood Serum Blood Tests Blood Vessels Cardiovascular Disease Cardiovascular System Disease Community Based Humans Intervention Laboratory Techniques Laboratory Testing Proteins Regression Analysis Risk Assessment Risk Factors Total Worker Health TWH Veins

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• ISSN:
  1948-5565
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; Massachusetts ; New York ; Oregon ; OSHA Region 1 ; OSHA Region 10 ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 5 ; Pennsylvania ; Rhode Island
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  285-297
• Volume:
  61
• Issue:
  3
• NIOSHTIC Number:
  nn:20049096
• Citation:
  Biodemography Soc Biol 2015 Dec; 61(3):285-297
• Contact Point Address:
  Orfeu M. Buxton, Department of Biobehavioral Health, 221 BBH Building, Pennsylvania State University, University Park, PA 16802, USA
• Email:
  Orfeu@PSU.edu
• Federal Fiscal Year:
  2016
• Performing Organization:
  Harvard University, Boston, Massachusetts
• Peer Reviewed:
  True
• Start Date:
  20070901
• Source Full Name:
  Biodemography and Social Biology
• End Date:
  20260831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6dde8057ad9503f1c7dfd46791b68b7829877fe3417739f1e2d9d14f989c279962c9136ca0645146c31f23d6a185b00d345db45d0f9f2542b6e38ad4a67e5b1c
• Download URL:
  https://stacks.cdc.gov/view/cdc/203669/cdc_203669_DS1.pdf
• File Type:
  [PDF - 1.06 MB ]