Benzyl butyl phthalate promotes adipogenesis in 3T3-L1 preadipocytes: a High Content Cellomics and metabolomic analysis

Details

• Personal Author:
  Lu K ; Yin L ; Yu KS ; Yu X
• Description:
  Benzyl butyl phthalate (BBP) has been known to induce developmental and reproductive toxicity. However, its association with dysregulation of adipogenesis has been poorly investigated. The present study aimed to examine the effect of BBP on the adipogenesis, and to elucidate the underlying mechanisms using the 3T3-L1 cells. The capacity of BBP to promote adipogenesis was evaluated by multiple staining approaches combined with a High Content Cellomics analysis. The dynamic changes of adipogenic regulatory genes and proteins were examined, and the metabolite profile was identified using GC/MC based metabolomic analysis. The High Content analysis showed BBP in contrast with Bisphenol A (BPA), a known environmental obesogen, increased lipid droplet accumulation in a similar dose-dependent manner. However, the size of the lipid droplets in BBP-treated cells was significantly larger than those in cells treated with BPA. BBP significantly induced mRNA expression of transcriptional factors C/EBPalpha and PPARgamma, their downstream genes, and numerous adipogenic proteins in a dose and time-dependent manner. Furthermore, GC/MC metabolomic analysis revealed that BBP exposure perturbed the metabolic profiles that are associated with glyceroneogenesis and fatty acid synthesis. Altogether, our current study clearly demonstrates that BBP promoted the differentiation of 3T3-L1 through the activation of the adipogenic pathway and metabolic disturbance. [Description provided by NIOSH]
• Subjects:
  Energy Metabolism Lipids Occupational Health Phthalic Acids Safety Toxicology
• Keywords:
  Adipogenesis Author Keywords: Benzyl Butyl Phthalate Bioinformatics Dose Response Fatty Acids Gene Expression HCA High Content Cellomics Analysis In Vitro Study Metabolic Effects Metabolism Metabolomic Analysis Obesity Phthalates

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• ISSN:
  0887-2333
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Georgia ; OSHA Region 4 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  297-309
• Volume:
  32
• NIOSHTIC Number:
  nn:20049092
• Citation:
  Toxicol In Vitro 2016 Apr; 32:297-309
• Contact Point Address:
  Xiaozhong Yu, Department of Environmental Health Science, College of Public Health, University of Georgia, EHS Bldg., 150 Green Street, Athens, GA 30602, United States
• Email:
  yuxz@uga.edu
• CAS Registry Number:
  Butyl benzyl phthalate (CAS RN 85-68-7)
• Federal Fiscal Year:
  2016
• Performing Organization:
  University of Georgia, Athens
• Peer Reviewed:
  True
• Start Date:
  20130901
• Source Full Name:
  Toxicology in Vitro
• End Date:
  20160831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f10b69da8d3286fbe7b195cc2b75a5f758c4be3624da9442dd24eb470d121be419f65799a26a559b1e62acf606cafdc4a2178b4a57f65c44de55e40810b370e
• Download URL:
  https://stacks.cdc.gov/view/cdc/203665/cdc_203665_DS1.pdf
• File Type:
  [PDF - 2.53 MB ]