Gene expression profiling of the effects of organic dust in lung epithelial and THP-1 cells reveals inductive effects on inflammatory and immune response genes

Details

• Personal Author:
  Boggaram V ; Gottipati KR ; Loose DS ; Mitchell CT ; Natarajan K
• Description:
  The intensification and concentration of animal production operations expose workers to high levels of organic dusts in the work environment. Exposure to organic dusts is a risk factor for the development of acute and chronic respiratory symptoms and diseases. Lung epithelium plays important roles in the control of immune and inflammatory responses to environmental agents to maintain lung health. To better understand the effects of organic dust on lung inflammatory responses, we characterized the gene expression profiles of A549 alveolar and Beas2B bronchial epithelial and THP-1 monocytic cells influenced by exposure to poultry dust extract by DNA microarray analysis using Illumina Human HT-12 v4 Expression BeadChip. We found that A549 alveolar and Beas2B bronchial epithelial and THP-1 cells responded with unique changes in the gene expression profiles with regulation of genes encoding inflammatory cytokines, chemokines, and other inflammatory proteins being common to all the three cells. Significantly induced genes included IL-8, IL-6, IL-1β, ICAM-1, CCL2, CCL5, TLR4, and PTGS2. Validation by real-time qRT-PCR, ELISA, Western immunoblotting, and immunohistochemical staining of lung sections from mice exposed to dust extract validated DNA microarray results. Pathway analysis indicated that dust extract induced changes in gene expression influenced functions related to cellular growth and proliferation, cell death and survival, and cellular development. These data show that a broad range of inflammatory mediators produced in response to poultry dust exposure can modulate lung immune and inflammatory responses. This is the first report on organic dust induced changes in expression profiles in lung epithelial and THP-1 monocytic cells. [Description provided by NIOSH]
• Subjects:
  Agriculture Dust Enzymes Immune System Lung Diseases Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Agricultural Industry Author Keywords: Lung Epithelium Cytokines Dust Exposure Dust Inhalation ELISA Enzyme-linked Immunosorbent Assay Gene Expression Histochemical Analysis Immune Reaction Inflammation Lung Disease Occupational Lung Diseases Organic Dusts

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• ISSN:
  1094-8341
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  281-289
• Volume:
  48
• Issue:
  4
• NIOSHTIC Number:
  nn:20049057
• Citation:
  Physiol Genomics 2016 Apr; 48(4):281-289
• Contact Point Address:
  V. Boggaram, Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708-3154
• Email:
  vijay.boggaram@uthct.edu
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Texas Health Center at Tyler
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Physiological Genomics
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e216952e16a8b025537c99b353948446f84009c3a4b980b18f3b3554ad40818631d5933218151067e55d8b5626d4a7453e1a0d4d617e4373b0ba75938c8dbe5
• Download URL:
  https://stacks.cdc.gov/view/cdc/203637/cdc_203637_DS1.pdf
• File Type:
  [PDF - 1.28 MB ]