Short-term markers of DNA damage among roofers who work with hot asphalt

Details

• Personal Author:
  Brindley S ; Dooley G ; Gan R ; Juarez-colunga E ; Serdar B ; Volckens J
• Description:
  Background: Roofers are at increased risk for various malignancies and their occupational exposures to polycyclic aromatic hydrocarbons (PAHs) have been considered as important risk factors. The overall goal of this project was to investigate the usefulness of phosphorylated histone H2AX (yH2AX) as a short-term biomarker of DNA damage among roofers. Methods: Blood, urine, and dermal wipe samples were collected from 20 roofers who work with hot asphalt before and after 6 h of work on Monday and Thursday of the same week (4 sampling periods). Particle-bound and gas-phase PAHs were collected using personal monitors during work hours. yH2AX was quantified in peripheral lymphocytes using flow cytometry and 8-hydroxy-2-deoxyguanosine (8-OHdG) was assessed in urine using ELISA. General linear mixed models were used to evaluate associations between DNA damage and possible predictors (such as sampling period, exposure levels, work- and life-style factors). Differences in mean biomarker and DNA damage levels were tested via ANOVA contrasts. Results: Exposure measurements did not show an association with any of the urinary biomarkers or the measures of DNA damage. Naphthalene was the most abundant PAH in gas-phase, while benzo(e)pyrene was the most abundant particle-bound PAH. Post-shift levels of yH2AX and 8-OHdG were higher on both study days, when compared to pre-shift levels. Cigarette smoking was a predictor of yH2AX and urinary creatinine was a predictor of urinary 8-OHdG. Between-subject variance to total variance ratio was 35.3 % for yH2ax and 4.8 % for 8-OHdG. Conclusion: yH2AX is a promising biomarker of DNA damage in occupational epidemiology studies. It has a lower within-subject variation than urinary 8-OHdG and can easily be detected in large scale groups. Future studies that explore the kinetics of H2AX phosphorylation in relation to chemical exposures may reveal the transient and persistent nature of this sensitive biomarker of early DNA damage. [Description provided by NIOSH]
• Subjects:
  Benzopyrenes Biomarkers Blood Construction Industry Environmental Exposure Epidemiology Hydrocarbons Neoplasms Occupational Health Particulate Matter Polycyclic Aromatic Hydrocarbons Risk Assessment Risk Factors Safety Skin Smokers Urine Workplace

  More +
• Keywords:
  8-hydroxy-deoxyguanosine Asphalt Fume Asphalt Industry Author Keywords: Polycyclic Aromatic Hydrocarbons Dermal Exposures DNA Damage DNA Double Strand Breaks Exposure Levels Gases Lymphocytes Malignancy Models Monitoring Systems Monitors Naphthalene PAHs Particulates Polycyclic Aromatic Hydrocarbons Risk Factors Roofers Sampling Smoking Statistical Analysis Work Environment Workers YH2AX

  More +
• ISSN:
  1476-069X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  99
• Volume:
  15
• NIOSHTIC Number:
  nn:20049022
• Citation:
  Environ Health 2016 Oct; 15:99
• Contact Point Address:
  Berrin Serdar, Colorado School of Public Health, University of Colorado Anschutz Medical Campus, Mail Stop B119-V20, 12850 East Montview Boulevard, Rm. V20-3126, Aurora, CO 80045
• Email:
  Berrin.Serdar@ucdenver.edu
• CAS Registry Number:
  Benzo[e]pyrene (CAS RN 192-97-2) ; Naphthalene (CAS RN 91-20-3)
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of Colorado, Denver
• Peer Reviewed:
  True
• Start Date:
  20100901
• Source Full Name:
  Environmental Health: A Global Access Science Source
• End Date:
  20140831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:eea8484fbcea6892c807c5b95d92929d0461f167fe6053583aba0866d7a5228c4a4a5823d83e38004c9e6b3649fdbb65467e46a31a55450fcce2ff3dc5cb2238
• Download URL:
  https://stacks.cdc.gov/view/cdc/203616/cdc_203616_DS1.pdf
• File Type:
  [PDF - 820.11 KB ]