Evaluation of sampling methods for toxicological testing of indoor air particulate matter

Details

• Personal Author:
  Chen BT ; Hirvonen M-R ; Huttunen K ; Hyvarinen A ; Leppanen H ; Lindsley, William G. ; Taubel M ; Tirkkonen J ; Chen BT ; Hirvonen M-R ; Huttunen K ; Hyvarinen A ; Leppanen H ; Lindsley, William G. ; Taubel M ; Tirkkonen J
• Description:
  There is a need for toxicity tests capable of recognizing indoor environments with compromised air quality, especially in the context of moisture damage. One of the key issues is sampling, which should both provide meaningful material for analyses and fulfill requirements imposed by practitioners using toxicity tests for health risk assessment. We aimed to evaluate different existing methods of sampling indoor particulate matter (PM) to develop a suitable sampling strategy for a toxicological assay. During three sampling campaigns in moisture-damaged and non-damaged school buildings, we evaluated one passive and three active sampling methods: the Settled Dust Box (SDB), the Button Aerosol Sampler, the Harvard Impactor and the National Institute for Occupational Safety and Health (NIOSH) Bioaerosol Cyclone Sampler. Mouse RAW264.7 macrophages were exposed to particle suspensions and cell metabolic activity (CMA), production of nitric oxide (NO) and tumor necrosis factor (TNFa) were determined after 24 h of exposure. The repeatability of the toxicological analyses was very good for all tested sampler types. Variability within the schools was found to be high especially between different classrooms in the moisture-damaged school. Passively collected settled dust and PM collected actively with the NIOSH Sampler (Stage 1) caused a clear response in exposed cells. The results suggested the higher relative immunotoxicological activity of dust from the moisture-damaged school. The NIOSH Sampler is a promising candidate for the collection of size-fractionated PM to be used in toxicity testing. The applicability of such sampling strategy in grading moisture damage severity in buildings needs to be developed further in a larger cohort of buildings. [Description provided by NIOSH]
• Subjects:
  Air Pollution, Indoor Animals Dust Energy Metabolism Environmental Exposure Laboratories Neoplasms Nitric Oxide Occupational Health Particulate Matter Risk Assessment Risk Factors Safety Toxicology

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• Keywords:
  Author Keywords: Indoor Air Cell Function Dusts Exposure Levels IAQ IEQ Indoor Air Pollution Indoor Air Quality Indoor Environmental Quality Indoor Environments In Vitro Laboratory Animals Metabolic Activation Metabolic Effects Moisture Damage Nitric Oxide Particulate Matter Particulates Risk Assessment Risk Factors Sampling Sampling Methods Toxicity Testing Tumors

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• ISSN:
  0895-8378
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  28
• Issue:
  11
• NIOSHTIC Number:
  nn:20048602
• Citation:
  Inhal Toxicol 2016 Sep; 28(11):500-507
• Contact Point Address:
  Jenni Tirkkonen, MSc, Inhalation Toxicology Laboratory, Department of Environmental Science, University of Eastern Finland, PO Box 1627, Kuopio FI-70211, Finland
• Email:
  jenni.tirkkonen@uef.fi
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Healthcare and Social Assistance ; Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Inhalation Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f1ed86e42c154a446d3abb12370859f11c0c67b3e07621b39968980bb50ef18753446e4285ae39a8664b4391ba03464228e7ccfd7a1dc451e48350f0da03a30
• Download URL:
  https://stacks.cdc.gov/view/cdc/203321/cdc_203321_DS1.pdf
• File Type:
  [PDF - 793.99 KB ]