An animal model of adult-onset allergic asthma

Details

• Personal Author:
  Castaneda A ; Cho Y ; Espiritu I ; Kadir T ; Peake J ; Pinkerton KE ; Shin J ; Tornes P ; Uyeminami D ; Castaneda A ; Cho Y ; Espiritu I ; Kadir T ; Peake J ; Pinkerton KE ; Shin J ; Tornes P ; Uyeminami D
• Description:
  Rationale: The mouse is the most widely used animal model for allergic asthma, with numerous studies using ovalbumin to induce asthmas in young, juvenile mice. However, there is no established murine model for adult-onset asthma, a severe disease mainly affecting women and associated with a more rapid decline in lung function compared to childhood-onset asthma. House dust mite (HDM) is a ubiquitous allergen and can trigger allergic asthma. Using HDM to induce asthma in adult mice could provide a reasonable animal model for adult-onset allergic asthma. The goal of this study was to determine the degree of reactivity of young and mature female C57 Black/6 mice exposed to HDM. Methods: Three and nine month old female C57 Black/6 mice were randomized into two groups each, HDM (n=6-7) and PBS (phosphate buffered saline, n=5-6). An allergic response was induced via intranasal instillation. Sensitization was performed on days 1, 3, and 5, and challenge was performed on days 12, 13, and 14. On day 15, the mice underwent pulmonary function tests and methacholine challenges for EC200RL (effective concentration that leads to a two fold increase in resistance). Bronchoalveolar lavage (BAL) fluid was collected for cell differentials. The right lung lobes were fixed and stained for histopathology. Results:, Pulmonary Function: Nine month old mice exposed to HDM had significantly decreased inspiratory capacity, decreased compliance, increased elastance, and increased small airway/peripheral tissue resistance while central airway resistance was not significantly different. For three month old mice, only esosinophils were significantly increased. This demonstrates a strong inflammatory/allergic response to HDM. Histopathology: The HDM group showed marked perivascular inflammation in contract to the lungs of PBS controls. Conclusions: Sensitization and challenge with HDM created a significant reactive airway response in nine month old female C57 Black/6 mice, but not in 3 month old mice. Both ages showed similar degrees of lung inflammation. These changes are consistent with adult-onset allergy, and potentially serve as a model of adult-onset asthma. [Description provided by NIOSH]
• Subjects:
  Age Factors Animals Asthma Blood Dust Hypersensitivity Immune System Laboratories Lung Macrophages Methacholine Compounds Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Age Factors Airway Resistance Allergens Allergic Disorders Allergies Alveolar Cells Analytical Models Animal Studies Bronchial Asthma Dusts Histopathology Immune Reaction Laboratory Animals Lung Cells Lung Clearance Lung Function Lung Tissue Methacholines Models Pulmonary Function Pulmonary Function Tests Respiratory System Disorders Sensitization

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• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  California ; Missouri ; OSHA Region 7 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  191
• NIOSHTIC Number:
  nn:20048457
• Citation:
  Am J Respir Crit Care Med 2015 May; 191(Abstract Issue):A1363
• Contact Point Address:
  J. Shin, University of California, Davis, Davis, CA
• Email:
  jsh@ucdavis.edu
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Abstract Issue
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:49fd53fda25ab6db8fa8a282cf286456d56a69f944d1d6bf0aa79bcb2dc469f3db3ea0ab1e44cae142f0d167ceaa51317a16b6393959b91bcce030840af8cea6
• Download URL:
  https://stacks.cdc.gov/view/cdc/203208/cdc_203208_DS1.pdf
• File Type:
  [PDF - 148.23 KB ]