Mechanistic evaluation of oxidant generation and the development of inflammation after pulmonary exposure to metal-rich welding nanoparticles

Details

• Personal Author:
  Bishop LM ; Erdely A ; Eye T ; Farris B ; Kodali V ; Meighan T ; Roberts, Jennifer R. ; Salmen R ; Shoeb M ; Zeidler-Erdely P
• Description:
  Welding generates incidental nanoparticles composed of cytotoxic metals (e.g., Cr, Mn, Ni, Fe). The goal was to use both in vivo and in vitro methodologies to determine the mechanisms by which welding nanoparticles may damage the lungs. To assess lung injury/inflammation, Sprague-Dawley rats were treated by intratracheal instillation (ITI) with 2.0 mg/rat of gas metal arc-mild steel (GMA-MS) or manual metal arc-stainless steel (MMA-SS) welding particles. At 1, 3, and 10 d, bronchoalveolar lavage was performed. To determine the cytotoxicity of the welding nanoparticles, RAW264.7 macrophages were exposed to MMA-SS or GMA-MS particles for 24 hr (0-50 mg/mL), and ROS generation and activation of inflammatory markers were assessed. Particle metal composition and size distribution were characterized by ICP-AES and MOUDI, respectively. MMA-SS (41% Fe, 29% Cr, 17% Mn, 3% Ni) and GMA-MS (85% Fe, 14% Mn) were arranged as chain-like agglomerates (MMAD: 240 nm) of primary nanoparticles (approximately 20-50 nm). Lung injury (LDH) and inflammation (neutrophil influx) were increased with ITI MMA-SS treatment compared to saline control and GMA-MS. RAW264.7 cells treated with MMA-SS caused increases in ROS, lipid aldehyde (4-hydroxynonenal) production, and COX-2 protein/gene expression. MMA-SS exposure also increased in vitro and in vivo protein expression of Nrf2 and HO-1 and in vitro gene expression of Hmox1. Welding generates nanoparticles with different metal profiles depending on the process. Welders exposed to SS particles are at a greater risk due to the presence of specific metals (e.g., Cr, Ni), increased cytotoxicity, and enhanced oxidative stress. [Description provided by NIOSH]
• Subjects:
  Aldehydes Biomarkers Cells, Cultured Chemistry Chromium Ferrous Compounds Formaldehyde Hazardous Substances Immune System Laboratories Lipids Lung Lung Diseases Manganese Metals Nickel Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology Welding

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• Keywords:
  Alveolar Cells Arc Welding Author Keywords: In Vitro Toxicology Biological-effects Cell Cultures Cell Culture Techniques Cellular Reactions Chemical Properties Cytotoxins Exposure Assessment Gene Expression Health-hazards Immune Reaction In Vitro Study In Vivo Study In Vivo Toxicology Iron Laboratory Animals Laboratory Testing Lung Cells Lung Disorders Metal/Metal Oxide Nanomaterials Metal Oxides Nanomaterials Nanoparticles Nanotechnology Nanotoxicology Neutrophils Occupational Safety Oxidative Stress Physical Properties Proteins Reactive Oxygen Species Risk Assessment ROS Stainless Steel Ultrafine-particulates

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• Publisher:
  International Nanotoxicology Congress
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20048184
• Citation:
  nanoTOX 2016, Proceedings of the 8th International Nanotoxicology Congress, June 1-4, 2016, Boston, Massachusetts. Boston: MA: International Nanotoxicology Congress, 2016 Jun; :258
• CAS Registry Number:
  4-Hydroxynonenal (CAS RN 4-Hydroxynonenal (CAS RN 75899-68-2)) ; Chromium (CAS RN 7440-47-3) ; Iron (CAS RN 7439-89-6) ; Manganese (CAS RN 7439-96-5) ; Nickel (CAS RN 7440-02-0) ; Stainless Steel (CAS RN 12597-68-1)
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Construction
• Peer Reviewed:
  False
• Source Full Name:
  nanoTOX 2016, Proceedings of the 8th International Nanotoxicology Congress, June 1-4, 2016, Boston, Massachusetts
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f9f7380734f41fe137bd33324148363ee8e23382a246ecb44c42156a6e6d94b04b744201539a087ee3eba3d913eb1381cfc1af13a8553b7ef37f62ce6a409dd
• Download URL:
  https://stacks.cdc.gov/view/cdc/203005/cdc_203005_DS1.pdf
• File Type:
  [PDF - 324.77 KB ]