A role for regulatory T cells in a murine model of epicutaneous toluene diisocyanate sensitization

Details

• Personal Author:
  Anderson, Stacey E. ; Kashon ML ; Long CM ; Lukomska E ; Marshall NB ; Meade BJ ; Shane H
• Description:
  Toluene diisocyanate (TDI) is a leading cause of chemical-induced occupational asthma which impacts workers in a variety of industries worldwide. Recently, the robust regulatory potential of regulatory T cells (Tregs) has become apparent, including their functional role in the regulation of allergic disease; however, their function in TDI-induced sensitization has not been explored. To elucidate the kinetics, phenotype, and function of Tregsduring TDI sensitization, BALB/c mice were dermally exposed (on each ear) to a single application of TDI (0.5-4% v/v) or acetone vehicle and endpoints were evaluated via RT-PCR and flow cytometry. The draining lymph node (dLN) Tregpopulation expanded significantly 4, 7, and 9 days after single 4% TDI exposure. This population was identified using a variety of surface and intracellular markers and was found to be phenotypically heterogeneous based on increased expression of markers including CD103, CCR6, CTLA4, ICOS, and Neuropilin-1 during TDI sensitization. Tregsisolated from TDI-sensitized mice were significantly more suppressive compared to their control counterparts, further supporting a functional role for Tregsduring TDI sensitization. Lastly, Tregswere depleted prior to TDI sensitization and an intensified sensitization response was observed. Collectively, these data indicate that Tregsexhibit a functional role during TDI sensitization. Because the role of Tregsin TDI sensitization has not been previously elucidated, these data contribute to the understanding of the immunologic mechanisms of chemical induced allergic disease. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Hypersensitivity Immune System Isocyanates Laboratories Occupational Health Safety Skin Toluene
• Keywords:
  Allergic Reactions Author Keywords: Chemical Sensitization Chemical Allergy Chemical Hypersensitivity Dermal Exposures Diisocyanates Hypersensitivity Immune Reaction Isocyanate Laboratory Animals Regulatory T Cells TDI Toluenes

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  85-98
• Volume:
  152
• Issue:
  1
• NIOSHTIC Number:
  nn:20047999
• Citation:
  Toxicol Sci 2016 Jul; 152(1):85-98
• Contact Point Address:
  Carrie Mae Long, CDC-NIOSH, 1095 Willowdale Road, Mailstop 4020, Morgantown, WV 26505
• Email:
  CMLong@cdc.gov
• CAS Registry Number:
  Toluene diisocyanate (CAS RN 26471-62-5)
• Federal Fiscal Year:
  2016
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7d3dbfb2870d9232b36f653713a89f49bfdc7d8236f6ce4a10ac636198dc6c95239649df7a328b788da1c69b183a8e15b525d61330959c4043a544f2d81e813f
• Download URL:
  https://stacks.cdc.gov/view/cdc/202892/cdc_202892_DS1.pdf
• File Type:
  [PDF - 862.93 KB ]