Cellular and subcellular immunohistochemical localization and quantification of cadmium ions in wheat (Triticum aestivum)

Details

• Personal Author:
  Gao W ; Li QX ; Li Z ; Meng F ; Nan T ; Tan G ; Tan W ; Wang B ; Zhao H
• Description:
  The distribution of metallic ions in plant tissues is associated with their toxicity and is important for understanding mechanisms of toxicity tolerance. A quantitative histochemical method can help advance knowledge of cellular and subcellular localization and distribution of heavy metals in plant tissues. An immunohistochemical (IHC) imaging method for cadmium ions (Cd2+) was developed for the first time for the wheat Triticum aestivum grown in Cd2+-fortified soils. Also, 1-(4-Isothiocyanobenzyl)-ethylenediamine-N,N,N,N-tetraacetic acid (ITCB-EDTA) was used to chelate the mobile Cd2+. The ITCB-EDTA/Cd2+ complex was fixed with proteins in situ via the isothiocyano group. A new Cd2+-EDTA specific monoclonal antibody, 4F3B6D9A1, was used to locate the Cd2+-EDTA protein complex. After staining, the fluorescence intensities of sections of Cd2+-positive roots were compared with those of Cd2+-negative roots under a laser confocal scanning microscope, and the location of colloidal gold particles was determined with a transmission electron microscope. The results enable quantification of the Cd2+ content in plant tissues and illustrate Cd2+ translocation and cellular and subcellular responses of T. aestivum to Cd2+ stress. Compared to the conventional metal-S coprecipitation histochemical method, this new IHC method is quantitative, more specific and has less background interference. The subcellular location of Cd2+ was also confirmed with energy-dispersive X-ray microanalysis. The IHC method is suitable for locating and quantifying Cd2+ in plant tissues and can be extended to other heavy metallic ions. [Description provided by NIOSH]
• Subjects:
  Chemistry, Analytic Metals, Heavy Occupational Health Safety
• Keywords:
  Analytical Chemistry Analytical Processes Heavy Metals Histochemical Analysis Metallic Ions Plant Cells
• ISSN:
  1932-6203
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• Issue:
  5
• NIOSHTIC Number:
  nn:20047766
• Citation:
  PLoS One 2015 May; 10(5):e0123779
• Contact Point Address:
  Baomin Wang, College of Agronomy and Biotechnology, China Agricultural University, Beijing, China
• Email:
  wbaomin@263.net
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  PLoS One
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8a9998f630dd82abdbba0902bb902714ceb3f9dff82159a03862b8a838f32366708c13ad3fb73665125576efe70434e6a3e94bae1678bff9307c12201a692e86
• Download URL:
  https://stacks.cdc.gov/view/cdc/202752/cdc_202752_DS1.pdf
• File Type:
  [PDF - 6.36 MB ]