Increased serum TNF-alpha and matrix metalloproteinase-2 are associated with grip strength declines and tissue degeneration in a rat model of overuse

Details

• Personal Author:
  Barbe MF ; Barr AE ; Fisher PW ; Gao HGL ; Popoff SN ; Wade CK
• Description:
  We have shown that continued performance of repetitive tasks induces grip strength declines despite resolution of systemic inflammation. We hypothesize this is due to underlying tissue degeneration. Here, we assessed long term performance (18 weeks) of a high-repetition, low-force (HRLF) task in a rat model of reaching and grasping. We observed reduced grip strength immediately after training, and persistent grip strength declines in reach limbs of HRLF rats. Several inflammatory cytokines increased in serum of 6- and 12-week HRLF rats, e.g. tumor necrosis factor alpha (TNF-alpha). TNF- alpha was also increased in reach limb muscles and tendons at similar time points. A serum analyte of collagen degradation (matrix metalloproteinase-2, MMP2) was increased in serum of 18-week HRLF rats. MMP2 and several other MMPs, as well as two fibrogenic proteins (CTGF and TGFB1), were increased in 18-week HRLF tendons, which also showed histological signs of pathology. Thus, motor declines were associated earlier with tissue inflammation but later with tendon degenerative changes. Assaying for TNF- alpha and MMP2 provided important insights into the stages of inflammation and degradation in this model. [Description provided by NIOSH]
• Subjects:
  Mining Occupational Health Safety
• Keywords:
  Animals Laboratory Animals MSD Muscle Function Muscular Disorders Musculoskeletal Disorders Musculoskeletal System Disorders Pathology Proteins Repetitive Stress Tissue Culture

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• ISBN:
  9780945289418
• ISSN:
  1071-1813
• Publisher:
  Human Factors and Ergonomics Society
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Proceedings
• Place as Subject:
  Massachusetts ; Oregon ; OSHA Region 1 ; OSHA Region 10 ; OSHA Region 3 ; Pennsylvania
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  56
• NIOSHTIC Number:
  nn:20047720
• Citation:
  Proceedings of the Human Factors and Ergonomics Society 56th Annual Meeting, October 22-26, 2012, Boston, Massachusetts. Santa Monica, CA: Human Factors and Ergonomics Society, 2012 Sep; 56:743-747
• Contact Point Address:
  Helen G.L. Gao, Department of Anatomy and Cell Biology, Temple University School of Medicine, Philadelphia, PA 19140
• Federal Fiscal Year:
  2012
• Performing Organization:
  Temple University
• Peer Reviewed:
  True
• Start Date:
  20000601
• Source Full Name:
  Proceedings of the Human Factors and Ergonomics Society 56th Annual Meeting, October 22-26, 2012, Boston, Massachusetts
• End Date:
  20120731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:513eaa5f647be3a541eaaebed0f536c56b55846b615d01988bcadc3c00f696a69effb581aba7a53a3d0896479bb2ed849dff5ce83394880ebac15efad07436f0
• Download URL:
  https://stacks.cdc.gov/view/cdc/202718/cdc_202718_DS1.pdf
• File Type:
  [PDF - 779.02 KB ]