Neurotoxicity of trimethyltin in rat cochlear organotypic cultures

Details

• Personal Author:
  Ding D ; Roth JA ; Salvi R ; Sun H ; Yu J
• Description:
  Trimethyltin (TMT), which has a variety of applications in industry and agricultural, is a neurotoxin that is known to affect the auditory system as well as central nervous system of humans and experimental animals. However, the mechanisms underlying TMT-induced auditory dysfunction are poorly understood. To gain insights into the neurotoxic effect of TMT on the peripheral auditory system, we treated cochlear organotypic cultures with concentrations of TMT ranging from 5 to 100 microM for 24 h. Interestingly, TMT preferentially damaged auditory nerve fibers and spiral ganglion neurons in a dose-dependent manner, but had no noticeable effects on the sensory hair cells at the doses employed. TMT-induced damage to auditory neurons was associated with significant soma shrinkage, nuclear condensation, and activation of caspase-3, biomarkers indicative of apoptotic cell death. Our findings show that TMT is exclusively neurotoxicity in rat cochlear organotypic culture and that TMT-induced auditory neuron death occurs through a caspase-mediated apoptotic pathway. [Description provided by NIOSH]
• Subjects:
  Animals Biomarkers Cells, Cultured Ear Energy Metabolism Environmental Monitoring Laboratories Nervous System Nervous System Disorders Occupational Health Safety Sense Organs

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• Keywords:
  Apoptosis Auditory-system Author Keywords: Trimethyltin Caspase-3 Cell-alteration Cell-culture-techniques Cell-damage Cell-metabolism Central-nervous-system Central-nervous-system-disorders Cochlea Dose-response Ears Exposure-assessment Exposure-levels Laboratory-animals Laboratory-testing Metabolism Methyl-compounds Neurotoxic-effects Neurotoxicity Neurotoxins Ototoxicity Spiral Ganglion Neurons

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• ISSN:
  1029-8428
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  43-54
• Volume:
  28
• Issue:
  1
• NIOSHTIC Number:
  nn:20047524
• Citation:
  Neurotox Res 2015 Jul; 28(1):43-54
• Contact Point Address:
  Dalian Ding, Center for Hearing and Deafness, Sate University New York at Buffalo, Buffalo, NY 14214, USA
• Email:
  dding@buffalo.edu
• CAS Registry Number:
  Trimethylstannane (CAS RN 1631-73-8)
• Federal Fiscal Year:
  2015
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  State University of New York - Buffalo
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  Neurotoxicity Research
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b00562f1cdb3da667012cad6a9f371b6ec57a4d62df4a3b0cdba4cf24417c1b5c190f349e122e78985a06dc06ef26466d2353cb49a14b3c876e5bdd341162eb1
• Download URL:
  https://stacks.cdc.gov/view/cdc/202563/cdc_202563_DS1.pdf
• File Type:
  [PDF - 930.99 KB ]