Development and characterization of antibody reagents for detecting nanoparticles

Details

• Personal Author:
  Bentley KL ; Callahan LM ; DeLouise LA ; Ravichandran S ; Sullivan MA
• Description:
  The increasing use of nanoparticles (NPs) in technological applications and in commercial products has escalated environmental health and safety concerns. The detection of NPs in the environment and in biological systems is challenged by limitations associated with commonly used analytical techniques. In this paper we report on the development and characterization of NP binding antibodies, termed NProbes. Phage display methodology was used to discover antibodies that bind NPs dispersed in solution. We present a proof-of-concept for the generation of NProbes and their use for detecting quantum dots and titanium dioxide NPs in vitro and in an ex vivo human skin model. Continued development and refinement of NProbes to detect NPs that vary in composition, shape, size, and surface coating will comprise a powerful tool kit that can be used to advance nanotechnology research particularly in the nanotoxicology and nanotherapeutics fields. [Description provided by NIOSH]
• Subjects:
  Environmental Exposure Environmental Health Occupational Health Risk Assessment Risk Factors Safety Safety Management Skin
• Keywords:
  Biological-effects Biological-function Biological-systems Environmental-exposure Environmental-factors Environmental-health Exposure-levels Humans In-vitro-study In-vivo-studies Models Nanotechnology Risk-factors Safety-measures

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• ISSN:
  2040-3364
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; New York ; OSHA Region 2 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  7
• Issue:
  47
• NIOSHTIC Number:
  nn:20047342
• Citation:
  Nanoscale 2015 Dec; 7(47):20042-20054
• Contact Point Address:
  Lisa A. DeLouise, Department of Biomedical Engineering, University of Rochester, Rochester, New York 14642
• Email:
  Lisa_DeLouise@urmc.rochester.edu
• CAS Registry Number:
  Titania (CAS RN 13463-67-7)
• Federal Fiscal Year:
  2016
• Performing Organization:
  University of Rochester, New York
• Peer Reviewed:
  True
• Start Date:
  20110901
• Source Full Name:
  Nanoscale
• End Date:
  20140831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5892ad4271d5cfbaed6365b1e055f381bf33fa9e4bfe20bb90dc532c648e18b4c547fed820083fc8e73ad697389c0fb5a3fee64299b0790abd77e21b65168e1f
• Download URL:
  https://stacks.cdc.gov/view/cdc/202447/cdc_202447_DS1.pdf
• File Type:
  [PDF - 18.11 MB ]