Butter flavoring-elicited relaxation and bioelectric responses of rat airway are independent of bitter taste receptors (TAS2R)

Details

• Personal Author:
  Fedan, Jeffery S. ; Shimko M ; ThompsonJ ; Zaccone E
• Description:
  "Popcorn workers' lung" is a fixed obstructive pulmonary disease caused by inhalation of artificial butter flavoring vapor. In the isolated, perfused rat trachea preparation, the butter flavorings, diacetyl and 2,3-pentanedione elicit airway smooth muscle (ASM) relaxation. TAS2Rs mediate relaxation of ASM by increasing the concentration of intracellular Ca2+ via large-conductance Ca2+-activated K+ (BKCa) channels. This study investigated the potential involvement of TAS2Rs in relaxant responses of tracheal ASM and bioelectric responses of tracheal epithelial cells in response to denatonium (1 mM), a TAS2R agonist, diacetyl (3 mM) and 2,3-pentanedione (3 mM). In rat tracheal strips contracted with methacholine (3×10-5 M), denatonium-induced relaxation responses were inhibited by iberiotoxin (IbTx; 100 nM), a BKCa channel blocker, suggesting that denatonium-induced relaxation is mediated by TAS2R. However, relaxation responses to the butter flavorings were not antagonized by IbTx. Whether TAS2R mediate bioelectric responses to denatonium and flavorings in the epithelium was investigated. The decrease in short-circuit current (/sc) in response to denatonium and flavorings were not blocked by apical IbTx, suggesting that the responses in the epithelium were not mediated by TAS2R. Our findings indicate that BKCa channels are not involved in flavoring-induced bronchodilation or /sc responses in rat airways. [Description provided by NIOSH]
• Subjects:
  Animals Food Additives Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Solvents
• Keywords:
  Cell-function Cellular-function Diseases Food-additives Foodstuff Laboratory-animals Lung-disease Lung-function Nerve-function Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Vapors

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  28
• NIOSHTIC Number:
  nn:20046922
• Citation:
  FASEB J 2014 Apr; 28(Suppl 1):660.14
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:acbb73a71dfdffaaeceabb05cd474dd8431f14610406e1d473dd4bfdb15a9f4e62c81838ecd7e4886a53596206a383673f1b9cd75f13307d625759f501feeea1
• Download URL:
  https://stacks.cdc.gov/view/cdc/202185/cdc_202185_DS1.pdf
• File Type:
  [PDF - 58.82 KB ]