Transmission of H7N9 influenza viruses with a polymorphism at PB2 residue 627 in chickens and ferrets

Details

• Personal Author:
  Cheung PPH ; Choy K-T ; Ho CCK ; Ip S-M ; Lee EF ; Leung CYH ; Li PCH ; Lindsley, William G. ; Luk GSM ; Peiris M ; Poon LLM ; Sia SF ; Wai CKL ; Yen H-L ; Zhou J
• Description:
  Poultry exposure is a major risk factor for human H7N9 zoonotic infections of which the mode of transmission remains unclear. We studied the transmission of genetically related poultry and human H7N9 influenza viruses differed by four amino acids including the host determinant PB2 residue 627. A/Silkie Chicken/HK/1772/2014 (SCk1772) and A/HK/3263/14 (HK3263) replicated to comparable titers in chickens with superior oropharyngeal over cloacal shedding; both viruses transmitted efficiently among chickens via direct contact but inefficiently via airborne route. Interspecies transmission via the airborne route was observed for ferrets exposed to the SCk1772 or HK3263 infected chickens while low numbers of copies of influenza viral genome were detected in the air, predominantly at particle sizes larger than 4 microm. In ferrets, the human isolate HK3263 replicated to higher titers and transmitted more efficiently via direct contact than SCk1772. We monitored "intra-host" and "inter-host" adaptive changes at PB2 residue 627 during infection and transmission of the SCK1772 that carried E627 and HK3263 that carried V/K/E polymorphism at 60%, 20%, and 20%, respectively. For SCk1772, positive selection for K627 over E627 was observed in ferrets during the chicken-to-ferret or ferret-to-ferret transmission. For HK3263 that contained V/K/E polymorphism, mixed V627 and E627 genotypes were transmitted among chickens while either V627 or K627 was transmitted from chicken-to-ferret or ferret-to-ferret with a narrow transmission bottleneck. Overall, our results suggest direct contact as the main mode for H7N9 transmission and identify the PB2-V627 genotype with uncompromised fitness and transmissibility in both avian and mammalian species. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Amino Acids Animals Communicable Diseases Food Industry Genetics Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Viral Diseases

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• Keywords:
  Adaptation Airborne-particles Amino-acids Disease-transmission Genetic-factors Humans Infectious-diseases Morphology Oropharynx Poultry Respiratory-infections Respiratory-system-disorders Risk-factors Viral-diseases Viral-infections Zoonoses

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• ISSN:
  0022-538X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  89
• Issue:
  19
• NIOSHTIC Number:
  nn:20046616
• Citation:
  J Virology 2015 Oct; 89(19):9939-9951
• Contact Point Address:
  Hui-Ling Yen, School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong
• Email:
  hyen@hku.hk
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Virology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b2ddef1a0c1c9295e361051553d42b38cdbe2434b34fc294aa225bf1718927f2875784bb9ade4b9231d8cf9b25485a528cc38e927b2a5a90a03f3adce913b59e
• Download URL:
  https://stacks.cdc.gov/view/cdc/201618/cdc_201618_DS1.pdf
• File Type:
  [PDF - 4.34 MB ]