Equivalence of influenza A virus RNA recovery from nasal swabs when lysing the swab and storage medium versus storage medium alone

Details

• Personal Author:
  Heaney CD ; Nadimpalli M ; Pisanic N ; Stewart J
• Description:
  Surveillance of healthy individuals at high risk for zoonotic influenza A transmission is important for tracking trends in influenza A epidemiology. Practical measurement methods that maximize viral recovery and produce low variability are essential when low viral loads are expected. For this study, lysing both a nasal swab and its storage medium was compared to lysing the storage medium alone to determine which method results in greater influenza A virus recovery. Independent results from two laboratories suggest that including the swab in the lysis step does not lead to higher influenza A virus recovery, and that recovery is less variable when only the swab storage medium is extracted. These results indicate that simply lysing the swab storage medium is an effective extraction method for nasal swabs collected during studies of influenza A virus exposure among healthy populations. [Description provided by NIOSH]
• Subjects:
  Epidemiology Occupational Health Risk Assessment Risk Factors Safety Viral Diseases
• Keywords:
  Author Keywords: Influenza A Virus Diseases Exposure-levels Nasal Swabs Risk-factors Surveillance Surveillance-programs Viral-diseases Viral-infections Viral RNA Extraction
• ISSN:
  0166-0934
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Maryland ; North Carolina ; OSHA Region 3 ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  14-17
• Volume:
  217
• NIOSHTIC Number:
  nn:20046592
• Citation:
  J Virol Methods 2015 Jun; 217:14-17
• Contact Point Address:
  Maya Nadimpalli, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, 1301 Michael Hooker Research Center, 135 Dauer Drive, CB #7431, Chapel Hill, NC 27599
• Email:
  mnadim@live.unc.edu
• Federal Fiscal Year:
  2015
• Performing Organization:
  Johns Hopkins University, Baltimore, Maryland
• Peer Reviewed:
  True
• Start Date:
  20120701
• Source Full Name:
  Journal of Virological Methods
• End Date:
  20150630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0e0a82182a1cb3bea482628654158ee875a4fc644f89ed7bb0a95758ec8c00bb3151a0b09c99421ea3bfbb030a348e39a15f5277f61580a4ffa0be77e5a993e9
• Download URL:
  https://stacks.cdc.gov/view/cdc/201603/cdc_201603_DS1.pdf
• File Type:
  [PDF - 339.17 KB ]