Human sperm sex chromosome disomy and sperm DNA damage assessed by the neutral comet assay

Details

• Personal Author:
  Dadd R ; Korrick SA ; Marchetti F ; Martenies SE ; McAuliffe ME ; Perry MJ ; Williams PL
• Description:
  Study Question: Is there an association between human sperm sex chromosome disomy and sperm DNA damage? Summary Answer: An increase in human sperm XY disomy was associated with higher comet extent; however, there was no other consistent association of sex chromosome disomies with DNA damage. What is Known Already: There is limited published research on the association between sex chromosome disomy and sperm DNA damage and the findings are not consistent across studies. study design, size, and duration:We conducted a cross-sectional study of 190men(25% eversmoker,75%never smoker) from subfertile couples presenting at the Massachusetts General Hospital Fertility Clinic from January 2000 to May 2003. Participants/Materials, Setting, Methods: Multiprobe fluorescence in situ hybridization for chromosomes X,Yand 18 was used to determine XX, YY, XYand total sex chromosomedisomyin sperm nuclei using an automated scoring method.The neutral comet assay was used to measure sperm DNA damage, as reflected by comet extent, percentage DNA in the comet tail, and tail distributedmoment. Univariate and multiple linear regression models were constructed with sex chromosomedisomy (separate models for each of the four disomic conditions) as the independent variable, and DNA damage parameters (separate models for each measure of DNA damage) as the dependent variable. Main Results and the Role of Chance: Men with current or past smoking history had significantly greater comet extent (mm: regression coefficients with 95% CI) [XX18: 15.17 (1.98, 28.36); YY18: 14.68 (1.50, 27.86); XY18: 15.41 (2.37, 28.45); Total Sex Chromosome Disomy: 15.23 (2.09, 28.38)], and tail distributed moment [XX18: 3.01 (0.30, 5.72); YY18: 2.95 (0.24, 5.67); XY18: 3.04 (0.36, 5.72); Total Sex Chromosome Disomy: 3.10 (0.31, 5.71)] than men who had never smoked. In regression models adjusted for age and smoking, there was a positive association between XY disomy and comet extent. For an increase in XY disomy from 0.56 to 1.47% (representing the 25th to 75th percentile), there was a mean increase of 5.08 mm in comet extent. No other statistically significant findings were observed. Limitations, Reasons for Caution: A potential limitation of this study is that it is cross-sectional. Cross-sectional analyses by nature do not lend themselves to inference about directionality for any observed associations; therefore we cannot determine which variable is the cause and which one is the effect. A small sample size may be a further limitation. Comparison of these findings to other studies is limited due to methodological differences. Wider Implications of the Findings: Although consistent associations across sex chromosome disomies or DNAdamage measures were not observed, this study highlights the need to explore etiologies of sperm DNA damage and sex chromosomedisomy to better understand the potential mechanistic overlaps between the two. [Description provided by NIOSH]
• Subjects:
  Environmental Exposure Environmental Pollution Genetics Men Occupational Health Risk Assessment Risk Factors Safety Smokers
• Keywords:
  Aneuploidy Author Keywords: Fluorescence In Situ Hybridization DNA Damage Environmental-exposure Environmental-hazards Exposure-levels Genes Humans Neutral Comet Assay Risk-factors Semen Smoking Spermatozoa Statistical-analysis

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• ISSN:
  0268-1161
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  District of Columbia ; Massachusetts ; OSHA Region 1 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  29
• Issue:
  10
• NIOSHTIC Number:
  nn:20046323
• Citation:
  Hum Reprod 2015 Oct; 29(10):2148-2155
• Contact Point Address:
  M. J. Perry, Department of Environmental and Occupational Health, Milken Institute School of Public Health, The George Washington University, 950 New Hampshire Ave NW Suite 400, Washington, DC 20052
• Federal Fiscal Year:
  2016
• Performing Organization:
  Harvard School of Public Health
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Human Reproduction
• End Date:
  20280630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a1b43c992b9dcf02375eb1e8f1b0913ff7bd880dfe667a3ca1156e4396eef9f02d58eddc69b1cc95e30848b3d4fc9d86df0e6eb437866ad705793d393db96fa0
• Download URL:
  https://stacks.cdc.gov/view/cdc/201420/cdc_201420_DS1.pdf
• File Type:
  [PDF - 248.65 KB ]