Detection of 5-fluorouracil surface contamination in near real time

Details

• Personal Author:
  Connor, Thomas H. ; DeBord, D. Gayle ; Kurtz KS ; Pretty JR ; Robertson SA ; Sammons DL ; Smith JP ; Snawder, John E.
• Description:
  Objectives: Contamination of workplace surfaces by antineoplastic drugs presents an exposure risk for healthcare workers. Traditional instrumental methods to detect contamination such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) are sensitive and accurate but expensive and incapable of producing results in real time. This limits their utility in preventing worker exposure. We are currently developing monitors based on lateral flow immunoassay that can detect drug contamination in near real time. In this report, we describe the laboratory performance of a 5-fluorouracil (5-FU) monitor. Methods: The monitor was evaluated by spiking ceramic, vinyl, composite, stainless steel, and glass surfaces of 100 cm2 area with 5-FU masses of 0, 5, 10, 25, 50, and 100 ng. The surface was sampled with a wetted cotton swab, the swab was extracted with buffer, and the resulting solution was applied to a lateral flow monitor. Two ways of evaluating the response of these monitors were used: an electronic method where a lateral flow reader was used for measuring line intensities, and a visual method where the intensity of the test line was visually compared to the control line. Results: The 5-FU monitor is capable of detecting 10 ng/100 cm2 (0.1 ng/cm2) using the electronic reader and 25 ng/100 cm2 (0.25 ng/cm2) using the visual comparison method for the surfaces studied. The response of the monitors was compared to LC-MS/MS results for the same samples for validation and there was good correlation of the two methods but some differences in absolute response, especially at higher spiking levels for the surface samples. [Description provided by NIOSH]
• Subjects:
  Antineoplastic Agents Biopharmaceutics Carcinogens Chromatography, Gas Health Personnel Occupational Health Risk Assessment Risk Factors Safety Urogenital Diseases Urogenital System

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• Keywords:
  Antineoplastic-agents Author Keywords: Antineoplastic Drugs Direct Reading Drugs Exposure-levels Gas-liquid-chromatography Health-care-personnel Lateral Flow Medical-personnel Monitors Nursing Pharmaceuticals Reproductive-effects Reproductive-hazards Risk-factors Surface-properties

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• ISSN:
  1078-1552
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  396-408
• Volume:
  22
• Issue:
  3
• NIOSHTIC Number:
  nn:20046214
• Citation:
  J Oncol Pharm Pract 2016 Jun; 22(3):396-408
• Contact Point Address:
  Jerome P Smith, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, US Centers for Disease and Control Prevention, 1090 Tusculum Avenue, Cincinnati, OH 45226
• Email:
  jps3@cdc.gov
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Oncology Pharmacy Practice
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:90992ada0123c942187d3a37eabeb7db8b9e03d553d7c0fb6338edc3af48ee2590a6300b4231610ffb3ac2dd7e3440305523062c07b8f22420cca7402911564c
• Download URL:
  https://stacks.cdc.gov/view/cdc/201336/cdc_201336_DS1.pdf
• File Type:
  [PDF - 691.75 KB ]