Cellular localization and developmental changes of the different isoforms of divalent metal transporter 1 (DMT1) in the inner ear of rats

Details

• Personal Author:
  Ding D ; Roth JA ; Salvi R
• Description:
  Divalent metal transporter 1 (DMT1) is generally considered to be the major transmembrane protein responsible for the uptake of a variety of divalent cations. Four isoforms of DMT1 have been identified in mammalian cells encoded by a single gene that differ both in their N- and C-terminal sequences with two mRNA isoforms possessing an iron response element (IRE) motif downstream from the stop codon on the message. Two distinct promoter sites regulate production of the 1A or 1B isoforms (translation starts at exon 2) for both the +IRE or -IRE species of the transporter resulting in the generation of four distinct configurations of this protein. Prior studies from our laboratory using cochlear organotypic cultures isolated from postnatal day three rats (P3) have demonstrated that Mn causes significant and selective damage to sensory hair cells and auditory nerve fibers and spiral ganglion neurons in a time and concentration dependent manner. Since DMT1 plays a critical role in controlling the uptake of a variety of essential and toxic metals into the cochlea, we compared the distribution and developmental changes of the 1A, +IRE and -IRE isoforms in rat inner ear. Results reveal that all three isoforms of DMT1 are selectively expressed in different cell populations within the cochlea and, additionally, demonstrate their cellular and subcellular distribution changes with development. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Ear Energy Metabolism Genetics Laboratories Manganese Occupational Health Safety Sense Organs
• Keywords:
  Auditory-nerve Auditory-system Cell-function Cellular-function Chemical-composition Chemical-properties Drugs Ears Laboratory-animals Mammalian-cells Manganese-compounds Metabolism Pharmacology Proteins

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• ISSN:
  0966-0844
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  125-134
• Volume:
  27
• Issue:
  1
• NIOSHTIC Number:
  nn:20045760
• Citation:
  BioMetals 2014 Feb; 27(1):125-134
• Contact Point Address:
  D. Ding, Center for Hearing and Deafness, University at Buffalo, 137 Cary Hall, Buffalo, NY, 14214
• Email:
  dding@acsu.buffalo.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  State University of New York - Buffalo
• Peer Reviewed:
  True
• Start Date:
  20120901
• Source Full Name:
  BioMetals
• End Date:
  20170831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b81360799c2d3b763d91c2c364755404d4bfd9cadfec3f67936bc06c5991409b256bb07064a352b1b162dbcd7aa5ac0c917149dfcf58e529d9504650697cd005
• Download URL:
  https://stacks.cdc.gov/view/cdc/201053/cdc_201053_DS1.pdf
• File Type:
  [PDF - 1.03 MB ]