Effects of DNA repair gene polymorphisms on DNA damage in human lymphocytes induced by a vinyl chloride metabolite in vitro

Details

• Personal Author:
  Brandt-Rauf PW ; Feng N ; Li Y ; Long C ; Xia Z-L
• Description:
  Background: Epidemiologic studies suggest that variability in DNA damage from vinyl chloride monomer (VCM) may be partially mediated by genetic polymorphisms in DNA repair. This study aimed to corroborate these observations with controlled experiments in vitro using cell lines from individuals with differing DNA repair genotypes to determine damage following VCM metabolite exposure. Methods: Matched pairs of lymphoblast cell lines (homozygous wild-type versus homozygous variant for either XRCC1 399 or XPD 751 polymorphism) were exposed to chloroacetaldehyde and analyzed by the cytokinesis-block micronucleus assay. Results: All cell lines demonstrated a dose-response of increasing micronuclei with increasing exposure, but for both XRCC1 and XPD, the polymorphic cells peaked at higher micronucleus frequencies and declined at a slower rate to baseline than the wild-type cells. Conclusion: This supports the findings that XRCC1 and XPD polymorphisms may result in deficient DNA repair of VCM-induced genetic damage. [Description provided by NIOSH]
• Subjects:
  DNA Energy Metabolism Epidemiology Genetics Occupational Health Safety
• Keywords:
  Author Keywords: DNA Repair Cell-biology Cell-function Cell-metabolism Cellular-function DNA-damage Exposure-levels Genes In-vitro-study Metabolism Metabolites Micronuclei Monomers Morphology Polymorphisms

  More +
• ISSN:
  1354-750X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Illinois ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  281-286
• Volume:
  19
• Issue:
  4
• NIOSHTIC Number:
  nn:20045510
• Citation:
  Biomarkers 2014 Jun; 19(4):281-286
• Contact Point Address:
  Dr. Paul W. Brandt-Rauf, Dean's Office, School of Public Health, University of Illinois at Chicago, 1603 West Taylor Street, Chicago, IL 60612
• Email:
  pwb1@uic.edu
• CAS Registry Number:
  Chloroacetaldehyde (CAS RN 107-20-0) ; Poly(vinyl chloride) (CAS RN 9002-86-2) ; Vinyl chloride (CAS RN 75-01-4)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Illinois at Chicago
• Peer Reviewed:
  True
• Start Date:
  20010701
• Source Full Name:
  Biomarkers
• End Date:
  20150831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:397b4e52b5dbe3b1c29677908d55ae84707dfeb7a722916dee6f4fbd74af00e3c6662700e251714b738d232f8a9e09375cfe9711af3e2253702d27dbe5b12d7d
• Download URL:
  https://stacks.cdc.gov/view/cdc/200905/cdc_200905_DS1.pdf
• File Type:
  [PDF - 545.66 KB ]