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Improved spatial resolution of matrix-assisted laser desorption/ionization imaging of lipids in the brain by alkylated derivatives of 2,5-dihydroxybenzoic acid



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  • Personal Author:
  • Description:
    RATIONALE: Matrix-assisted laser desorption/ionization (MALDI) is one of the major techniques for mass spectrometry imaging (MSI) of biological systems along with secondary-ion mass spectrometry (SIMS) and desorption electrospray mass spectrometry (DESI). The inherent variability of MALDI-MSI signals within intact tissues is related to the heterogeneity of both the sample surface and the matrix crystallization. To circumvent some of these limitations of MALDI-MSI, we have developed improved matrices for lipid analysis based on structural modification of the commonly used matrix 2,5-dihydroxybenzoic acid (DHB). METHODS: We have synthesized DHB containing -C6H13 and -C12H25 alkyl chains and applied these matrices to rat brain using a capillary sprayer. We utilized a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer to analyze lipid extracts and tissue sections, and examined these sections with polarized light microscopy and differential interference contrast microscopy. RESULTS: O-alkylation of DHB yields matrices, which, when applied to brain sections, follow a trend of phase transition from crystals to an oily layer in the sequence DHB - DHB-C6H13 - DHB-C12H25 . MALDI-MSI images acquired with DHB-C12H25 exhibited a considerably higher density of lipids than DHB. CONCLUSIONS: Comparative experiments with DHB and DHB-C12H25 are presented, which indicate that the latter matrix affords higher lateral resolution than the former. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    0951-4198
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    403-412
  • Volume:
    28
  • Issue:
    5
  • NIOSHTIC Number:
    nn:20045110
  • Citation:
    Rapid Commun Mass Spectrom 2014 Mar; 28(5):403-412
  • Contact Point Address:
    V. E. Kagan, Department of Environmental and Occupational Health, University of Pittsburgh, 100 Technology Drive, Pittsburgh, PA 15219
  • Email:
    kagan@pitt.edu
  • Federal Fiscal Year:
    2014
  • NORA Priority Area:
    Manufacturing
  • Performing Organization:
    University of Pittsburgh at Pittsburgh
  • Peer Reviewed:
    True
  • Start Date:
    20050701
  • Source Full Name:
    Rapid Communications in Mass Spectrometry
  • End Date:
    20160630
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:9071db6b2c5e4cc822bd2614ee6ec1e5f4c1c2a47800519c210e5ef69524f135cb3293dbca302ad2b8d066b4a0ce728e6a403b4cc898eeb28951afa5d7456282
  • Download URL:
  • File Type:
    Filetype[PDF - 1.74 MB ]
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