Domestic exposure to fungal allergenic particles determined by halogen immunoassay using subject's serum versus particles carrying three non-fungal allergens determined by allergen-specific HIA

Details

• Personal Author:
  Green, Brett J. ; Liu-Brennan D ; McKay KO ; Sercombe JK ; Tovey ER
• Description:
  Studies that estimate indoor aeroallergen exposure typically measure a pre-selected limited range of allergens. In this study, inhalable aeroallergen particles were quantified using the halogen immunoassay (HIA) to determine the contribution of fungal and non-fungal aeroallergens to total allergen exposure. Bioaerosols from 39 homes of fungal-allergic subjects were sampled using inhalable fraction samplers and immunostained by HIA using resident subject's immunoglobulin E (IgE) to detect allergen-laden particles. Fungal aerosols as well as particles carrying mite, cat, and cockroach allergens were identified and enumerated by HIA. Reservoir dust-mite (Der p 1), cat (Fel d 1), and cockroach (Bla g 1) allergen concentrations were quantified by ELISA. Fungal particles that bound subject's IgE in the HIA were 1.7 (bedroom)- and 1.4 (living room)-fold more concentrated than Der p 1, Fel d 1, and Bla g 1 allergen particles combined. Predominant fungal conidia that bound IgE were derived from common environmental genera including Cladosporium and other fungi that produce amerospores. Airborne mite, cat, and cockroach allergen particle counts were not associated with reservoir concentrations determined by ELISA. This study demonstrates that inhalable fungal aerosols are the predominant aeroallergen sources in Sydney homes and should be considered in future exposure assessments. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollution Air Pollution, Indoor Environmental Health Fungi Hypersensitivity Occupational Health Particulate Matter Risk Assessment Risk Factors Safety

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• Keywords:
  Air-quality Airborne Fungi Allergen Exposure Allergens Allergic-reactions Allergies Author Keywords: Aeroallergens Bioaerosols Exposure-levels Fungal Allergens Indoor-environmental-quality Particulates Risk-factors Samplers

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• ISSN:
  0905-6947
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  438-445
• Volume:
  24
• Issue:
  4
• NIOSHTIC Number:
  nn:20044925
• Citation:
  Indoor Air 2014 Aug; 24(4):438-445
• Contact Point Address:
  J. K. Sercombe, Woolcock Institute of Medical Research, Rm 630 Blackburn Bld D06, The University of Sydney, 2006, Sydney, Australia
• Email:
  jason.sercombe@sydney.edu.au
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Indoor Air
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4b2d10f516355bbb6035c17df5a8c60e0477bd4d9a6751f6b8db141caeadf4795fd1bb2b2deae68ec5fbdd50c4eb6cb064a25921e6ac6c1b81a0f269c347221e
• Download URL:
  https://stacks.cdc.gov/view/cdc/200511/cdc_200511_DS1.pdf
• File Type:
  [PDF - 211.69 KB ]