The association between global DNA methylation and telomere length in a longitudinal study of boilermakers

Details

• Personal Author:
  Cavallari J ; Christiani, David C. ; De-Vivo I ; Grashow R ; Lin X ; Wong JYY
• Description:
  The objectives of this study were to determine if global DNA methylation, as reflected in LINE-1 and Alu elements, is associated with telomere length and whether it modifies the rate of telomeric change. A repeated-measures longitudinal study was performed with a panel of 87 boilermaker subjects. The follow-up period was 29 months. LINE-1 and Alu methylation was determined using pyrosequencing. Leukocyte relative telomere length was assessed via real-time qPCR. Linear-mixed models were used to estimate the association between DNA methylation and telomere length. A structural equation model (SEM) was used to explore the hypothesized relationship between DNA methylation, proxies of particulate matter exposure, and telomere length at baseline. There appeared to be a positive association between both LINE-1 and Alu methylation levels, and telomere length. For every incremental increase in LINE-1 methylation, there was a statistically significant 1.0 × 10(-1) (95% CI: 4.6 × 10(-2), 1.5 × 10(-1), P < 0.01) unit increase in relative telomere length, controlling for age at baseline, current and past smoking status, work history, BMI (log kg/m(2) ) and leukocyte differentials. Furthermore, for every incremental increase in Alu methylation, there was a statistically significant 6.2 × 10(-2) (95% CI: 1.0 × 10(-2), 1.1 × 10(-1), P = 0.02) unit increase in relative telomere length. The interaction between LINE-1 methylation and follow-up time was statistically significant with an estimate -9.8 × 10(-3) (95% CI: -1.8 × 10(-2), -1.9 × 10(-3), P = 0.02); suggesting that the rate of telomeric change was modified by the degree of LINE-1 methylation. No statistically significant association was found between the cumulative PM exposure construct, with global DNA methylation and telomere length at baseline. [Description provided by NIOSH]
• Subjects:
  Cardiovascular Diseases Cardiovascular System Cell Division Chemistry Energy Metabolism Epidemiology Genetics Men Metals Neoplasms Occupational Health Particulate Matter Safety

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• Keywords:
  Alu Author Keywords: Telomere Cancer Cardiovascular-disease Cell-division Chemical-composition Chemical-properties DNA Methylation Drugs Humans Leukocytes LINE-1 Longitudinal Metabolism Metal-compounds Metallic-compounds Particulates Pharmacology Proteins

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• ISSN:
  0741-0395
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Maryland ; Massachusetts ; OSHA Region 1 ; OSHA Region 3
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  254-264
• Volume:
  38
• Issue:
  3
• NIOSHTIC Number:
  nn:20044568
• Citation:
  Genet Epidemiol 2014 Apr; 38(3):254-264
• Contact Point Address:
  Jason Y. Y. Wong, Departments of Epidemiology and Environmental Health, Harvard School of Public Health, Channing Division ofNetwork Medicine, 181 Longwood Avenue, Boston, MA 02115
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Construction
• Performing Organization:
  CPWR - The Center for Construction Research and Training, Silver Spring, Maryland
• Peer Reviewed:
  True
• Start Date:
  20090901
• Source Full Name:
  Genetic Epidemiology
• End Date:
  20240831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c8d520e43983433829eab9d444bd64820c9a21d54d24de57cec5dd207c967114e87a5ab00ad325d6193e3028d7818b8f62047aa071b01b4f4f90bf50e87a8ecb
• Download URL:
  https://stacks.cdc.gov/view/cdc/200242/cdc_200242_DS1.pdf
• File Type:
  [PDF - 253.21 KB ]