The effects of perinatal exposure to secondhand smoke on the pulmonary immune response to allergens

Details

• Personal Author:
  Castaneda A ; Hassan M ; Pinkerton KE ; Castaneda A ; Hassan M ; Pinkerton KE
• Description:
  Rational: Prenatal and early postnatal exposure to secondhand tobacco smoke (TS) in mice enhances allergic airway inflammation by affecting the development of the immune system in the lung, exacerbating airway inflammation and hyper-reactivity. Ambient particulate matter (PM) acts as an adjuvant, enhancing the allergic inflammatory responses, however the combined effects of PM and TS on allergic airway inflammation have not been studied. Objective: To study how in-utero and postnatal TS exposure in mice shapes the airway immune response to the combined exposure of ovalbumin (OVA) and ambient PM. Methods: Time pregnant BALB/c mice were exposed to filtered air (FA) or 1 mg/m3 of TS (6 hrs/day, for 5 days). Newborn male offspring from FA exposed mothers were exposed to filtered air for 8 weeks, followed by exposure to PBS (n=6), OVA (n=6), PM (n=6), or OVA+PM (n=6). Newborn male offspring from TS-exposed mothers were further exposed to TS for 8 weeks, followed by exposure to PBS vehicle (n=6), OVA (n=6), PM (n=6), or OVA+PM (n=6). Lung tissue, bronchoalveolar lavage fluid (BALF) and plasma were analyzed for extent of inflammation, cellular infiltrates, cytokine and immunoglobulin E (IgE) levels. Results: In the FA model, OVA+PM significantly increased total cells in BALF compared to OVA alone. Eosinophils recovered in BALF and plasma IgE levels were significantly elevated in FA treatment groups compared to TS groups, indicating smoke suppressed Th2-mediated immune responses in our model. Both IgE and TNFá levels were significantly elevated in OVA and OVA+PM groups over their respective PBS controls in FA exposed mice, whereas OVA and OVA+PM groups were not significantly different from PBS controls in TS exposed mice. Conclusion: Our findings suggest prenatal and postnatal exposure to TS shape the development of the immune system in the lungs at an early age producing an immunosuppressive effect in response to allergic challenge in BALB/c mice. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Exposure Environmental Monitoring Hypersensitivity Immune System Laboratories Lung Diseases Occupational Health Particulate Matter Respiratory Tract Diseases Safety Smoke Smokers Tobacco Products

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• Keywords:
  Airway-obstruction Allergens Animal-studies Cigarette-smoking Environmental-exposure Exposure-assessment Exposure-levels Immune-reaction Immune-system Immune-system-disorders In-utero-exposure In-utero-study Laboratory-animals Lung-disorders Particulates Prenatal-exposure Pulmonary-system-disorders Respiratory-system-disorders Smoke-inhalation Tobacco-smoke

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• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  189
• NIOSHTIC Number:
  nn:20044480
• Citation:
  Am J Respir Crit Care Med 2014 May; 189(Meeting Abstracts):A4283
• Contact Point Address:
  A. Castaneda, University of California, Davis, CA
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Agriculture, Forestry and Fishing ; Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  False
• Start Date:
  20010930
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Meeting Abstracts
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0d08d440d2e5c4ccde668026515b1e33cafe503b3f08f5edd37ad79a3b76620abafd637c95354bef2c8195dd1706af9a3268bc4b28f3811e846d449cfc3c1e53
• Download URL:
  https://stacks.cdc.gov/view/cdc/200170/cdc_200170_DS1.pdf
• File Type:
  [PDF - 52.83 KB ]