Cellular interactions and biological responses to titanium dioxide nanoparticles in HepG2 and BEAS-2B cells: role of cell culture media

Details

• Personal Author:
  Blackman CF ; Demarini DM ; Fry RC ; Killius MG ; Kligerman AD ; Prasad RY ; Simmons SO ; Zucker RM
• Description:
  We showed previously that exposure of human lung cells (BEAS-2B) to TiO2 nanoparticles (nano-TiO2 ) produced micronuclei (MN) only when the final concentration of protein in the cell-culture medium was at least 1%. Nanoparticles localize in the liver; thus, we exposed human liver cells (HepG2) to nano-TiO2 and found the same requirement for MN induction. Nano-TiO2 also formed small agglomerates in medium containing as little as 1% protein and caused cellular interaction as measured by side scatter by flow cytometry and DNA damage (comet assay) in HepG2 cells. Nano-TiO2 also increased the activity of the inflammatory factor NFkB but not of AP1 in a reporter-gene HepG2 cell line. Suspension of nano-TiO2 in medium containing 0.1% protein was sufficient for induction of MN by the nanoparticles in either BEAS-2B or HepG2 cells as long the final concentration of protein in the cell-culture medium was at least 1%. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Cytology Energy Metabolism Lung Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Micronucleus Biological-effects Cell-cultures Cell-function Cellular-function Cellular-reactions Comet Assay Exposure-levels Flow Cytometry Humans Lung-cells Metabolism Nanotechnology Pharmacology Proteins

  More +
• ISSN:
  0893-6692
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  336-342
• Volume:
  55
• Issue:
  4
• NIOSHTIC Number:
  nn:20044361
• Citation:
  Environ Mol Mutagen 2014 May; 55(4):336-342
• Contact Point Address:
  David M. DeMarini, U.S. EPA, B105-03, RTP, NC 27711
• Email:
  demarini.david@epa.gov
• CAS Registry Number:
  Titanium (CAS RN 7440-32-6)
• Federal Fiscal Year:
  2014
• Performing Organization:
  University of North Carolina, Chapel Hill
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Environmental and Molecular Mutagenesis
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f7541304d7b0f249e31a04571c443434578a6ccd36d2cd81a17316f6464f8b5b4e385feac6da595ad50b120ce0188aa81d45d381c4117708c02de875ff31aa2
• Download URL:
  https://stacks.cdc.gov/view/cdc/200077/cdc_200077_DS1.pdf
• File Type:
  [PDF - 235.24 KB ]