Estimating the effective density of engineered nanomaterials for in vitro dosimetry

Details

• Personal Author:
  Cohen JM ; Darrah T ; DeLoid G ; Demokritou P ; Derk R ; Pyrgiotakis G ; Rojanasakul L ; Wohlleben W
• Description:
  The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. Here we present a rapid and inexpensive method for accurately measuring the effective density of nano-agglomerates in suspension. This novel method is based on the volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed cell volume tube, and is validated against gold-standard analytical ultracentrifugation data. This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano-bio interactions in vitro. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Occupational Health Particulate Matter Radiation Dosimeters Safety Toxicology
• Keywords:
  Cell-culture-techniques Dosimetry Exposure-levels In-vitro-study Models Nanotechnology Particulates
• ISSN:
  2041-1723
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  5
• NIOSHTIC Number:
  nn:20044300
• Citation:
  Nat Commun 2014 Mar; 5:3514
• Contact Point Address:
  Glen DeLoid, Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Ctr Nanotechnol & Nanotoxicol, 655 Huntington Ave, Boston, MA 02115
• Email:
  gdeloid@hsph.harvard.edu
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Nature Communications
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d6217a6b11c697e71f68ceae233e771d168f219112384a5fc35c9a3cb0a08601182a3916e6426de4a8dc154d398ab714a4f0cc718004078d1f4e176d2d102975
• Download URL:
  https://stacks.cdc.gov/view/cdc/200036/cdc_200036_DS1.pdf
• File Type:
  [PDF - 497.60 KB ]