Effect of fiber length on carbon nanotube-induced fibrogenesis

Details

• Personal Author:
  Battelli L ; Derk R ; Dinu CZ ; Dong C ; Guo H ; He X ; Luanpitpong S ; Manke A ; Mercer RR ; Porter DW ; Rojanasakul Y ; Sager T ; Stueckle, Todd A. ; Wang L ; Wu N
• Description:
  Given their extremely small size and light weight, carbon nanotubes (CNTs) can be readily inhaled by human lungs resulting in increased rates of pulmonary disorders, particularly fibrosis. Although the fibrogenic potential of CNTs is well established, there is a lack of consensus regarding the contribution of physicochemical attributes of CNTs on the underlying fibrotic outcome. We designed an experimentally validated in vitro fibroblast culture model aimed at investigating the effect of fiber length on single-walled CNT (SWCNT)-induced pulmonary fibrosis. The fibrogenic response to short and long SWCNTs was assessed via oxidative stress generation, collagen expression and transforming growth factor-beta (TGF-beta) production as potential fibrosis biomarkers. Long SWCNTs were significantly more potent than short SWCNTs in terms of reactive oxygen species (ROS) response, collagen production and TGF-beta release. Furthermore, our finding on the length-dependent in vitro fibrogenic response was validated by the in vivo lung fibrosis outcome, thus supporting the predictive value of the in vitro model. Our results also demonstrated the key role of ROS in SWCNT-induced collagen expression and TGF-beta activation, indicating the potential mechanisms of length-dependent SWCNT-induced fibrosis. Together, our study provides new evidence for the role of fiber length in SWCNT-induced lung fibrosis and offers a rapid cell-based assay for fibrogenicity testing of nanomaterials with the ability to predict pulmonary fibrogenic response in vivo. [Description provided by NIOSH]
• Subjects:
  Biological Assay Biomarkers Cells, Cultured Chemistry Lung Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Author Keywords: Carbon Nanotubes Bioassays Carbon-compounds Cell-alteration Cell-cultures Cellular-reactions Chemical-properties Fiber Length Fibrogenesis Fibrogenicity Fibrous-bodies In-vitro-study Lung-fibrosis Lung Fibrosis Molecular-structure Nanotechnology Oxidative-processes Physical-properties Pulmonary-system-disorders Respiratory-system-disorders ROS TGF-beta Type I Collagen

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• ISSN:
  1422-0067
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  15
• Issue:
  5
• NIOSHTIC Number:
  nn:20044249
• Citation:
  Int J Mol Sci 2014 Apr; 15(5):7444-7461
• Contact Point Address:
  Yon Rojanasakul, Department of Pharmaceutical Sciences, West Virginia University, 1 Medical Center Drive, Morgantown, WV 26506, USA
• Email:
  yrojan@hsc.wvu.edu
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2014
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  International Journal of Molecular Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:85ad9a49b8be5843b82e4f12ee79a53c94f1605332be8befc770da9dcf78a3ec3db18b9e74032df2fdc303cf63e9c409b4fae32e296cb867eae9ac085ec17f12
• Download URL:
  https://stacks.cdc.gov/view/cdc/199994/cdc_199994_DS1.pdf
• File Type:
  [PDF - 1.29 MB ]