Increased mechano-growth factor gene expression following stretch-shortening contraction loading: impact of glutathione and age

Details

• Personal Author:
  Baker BA ; Cutlip, Robert G. ; Ensey J ; Hollander MS ; Kashon ML
• Description:
  Specific genes regulating multiple pathways in the adaptive response in skeletal muscle following glutathione modulation and repetitive mechanical loading are not known. PURPOSE: The purpose of this study was to characterize gene transcripts influenced by dietary supplementation with a glutathione antagonist (L-Buthionine Sulfoximine (BSO)) following chronic high-intensity mechanical loading via stretch-shortening contractions (SSCs) in young and old rats. METHODS: Left dorsiflexor muscles of young (12 weeks, N= 32) and old (30 months, N = 30), vehicle- (VEH) and BSO-treated rats were exposed 3 times per week for 4.5-weeks to a protocol of 80 maximal SSCs per exposure in vivo, while cage, age-matched rats served as controls (CON). Messenger levels for MyoD, Bax, Bcl2, Caspase-9, IL-15, IL1-b, MCP-1, TGF-b1, IL-10, mechano-growth factor (MGF), and 18s (control) genes were characterized by RT-PCR analysis following the SSC-exposure period. RESULTS: Following 4.5 weeks of SSC loading only MGF mRNA levels were significantly altered by treatment (young BSO increased versus young VEH; p < 0.05) and age (young BSO increased versus old BSO; p < 0.05). CONCLUSIONS: Even though MGF mRNA was the only transcript influenced by glutathione modulation and/or aging, further investigation is needed to evaluate changes in protein levels. However, the present study clearly demonstrates that MGF mRNA is influenced by both glutathione modulation and aging. [Description provided by NIOSH]
• Subjects:
  Aging Animals Genetics Laboratories Musculoskeletal System Occupational Health Safety
• Keywords:
  Age-groups Genes Laboratory-animals Muscle-function Muscle-physiology Muscles Musculoskeletal-system
• ISSN:
  0195-9131
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  41
• Issue:
  5
• NIOSHTIC Number:
  nn:20044025
• Citation:
  Med Sci Sports Exerc 2009 May; 41(5)(Suppl 1):350
• Email:
  rgc8@CDC.GOV
• CAS Registry Number:
  Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  2009
• Peer Reviewed:
  False
• Source Full Name:
  Medicine and Science in Sports and Exercise
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ecb47c0a23c2c1b3a4b1777d13fcceb4c0442d57fb9e1eb63631efa9b663c230924e3b5badcd0fb472d481fd052524bdf977af4bb58a9cb21f2e2f6ebebd087d
• Download URL:
  https://stacks.cdc.gov/view/cdc/199879/cdc_199879_DS1.pdf
• File Type:
  [PDF - 43.88 KB ]