Characterization of damaged skin by impedance spectroscopy: chemical damage by dimethyl sulfoxide

Details

• Personal Author:
  Bunge AL ; Orazem ME ; White EA
• Description:
  PURPOSE: To relate changes in the electrochemical impedance spectra to the progression and mechanism of skin damage arising from exposure to dimethyl sulfoxide (DMSO). METHODS: Electrochemical impedance spectra measured before and after human cadaver skin was treated with neat DMSO or phosphate buffered saline (control) for 1 h or less were compared with electrical circuit models representing two contrasting theories describing the progression of DMSO damage. Flux of a model lipophilic compound (p-chloronitrobenzene) was also measured. RESULTS: The impedance spectra collected before and after 1 h treatment with DMSO were consistent with a single circuit model; whereas, the spectra collected after DMSO exposure for 0.25 h were consistent with the model circuits observed before and after DMSO treatment for 1 h combined in series. DMSO treatments did not significantly change the flux of p-chloronitrobenzene compared to control. CONCLUSIONS: Impedance measurements of human skin exposed to DMSO for less than about 0.5 h were consistent with the presence of two layers: one damaged irreversibly and one unchanged. The thickness of the damaged layer increased proportional to the square-root of treatment time until about 0.5 h, when DMSO affected the entire stratum corneum. Irreversible DMSO damage altered the lipophilic permeation pathway minimally. [Description provided by NIOSH]
• Subjects:
  Biological Factors Chemistry Electricity Energy Metabolism Hazardous Substances Occupational Health Pathology Phenol Risk Assessment Risk Factors Safety Skin Skin Absorption

  More +
• Keywords:
  Biological-factors Chemical-composition Chemical-properties Drugs Electrical-fields Electrochemistry Exposure-levels Metabolism Models Phenols Risk-factors Skin-absorption Skin-exposure Spectroscopes Toxins

  More +
• ISSN:
  0724-8741
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  30
• Issue:
  10
• NIOSHTIC Number:
  nn:20043966
• Citation:
  Pharm Res 2013 Oct; 30(10):2607-2624
• CAS Registry Number:
  Dimethyl sulfoxide (CAS RN 67-68-5)
• Federal Fiscal Year:
  2014
• Performing Organization:
  Colorado School of Mines
• Peer Reviewed:
  True
• Start Date:
  20040901
• Source Full Name:
  Pharmaceutical Research
• End Date:
  20090331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2ad20d13abfc69201197c07373bf7c456ac35f12d8720dbcd0b2efcbaff80f2225ede26e4075b1122a2c0d22d4f821f13ea20625386be137a3815cc513d8a008
• Download URL:
  https://stacks.cdc.gov/view/cdc/199827/cdc_199827_DS1.pdf
• File Type:
  [PDF - 782.23 KB ]