Enhanced Nitric Oxide Production Associated with Silica-Induced Disease in Rats

Details

• Personal Author:
  Castranova, Vincent ; Hubbs, Ann F. ; Khan A ; McLaurin J ; Millecchia L ; Porter DW ; Ramsey D ; Robinson VA ; Teass A ; Willard P
• Description:
  This study investigated the relationship between silica-induced lung damage, inflammation and fibrosis and nitric oxide (NO) production in rats exposed by inhalation to silica (15 mgim3 silica, 6 hr/day, 5 days/week, for 116 exposure days). Markers of pulmonary inflammation and damage rise rapidly (5-16 days of exposure) and are maintained at a relatively stable new set point through 40 days, before rising explosively at 79 and 116 days of exposure. Fibrosis is evident only during this explosive period of inflammation and damage. Silica inhalation results in a rapid (5 days) rise in NO-dependent chemiluminescence from alveolar macrophages (AM) NO products in bronchoalveolar lavage fluid increase significantly, exhibiting a time course similar to that for parameters of inflammation and damage. Immunohistochemical analysis of pulmonary inducible NO synthase (iNOS) and nitrotyrosine identified elevated iNOS and NO- induced damage in AM and type II cells after 79 days of exposure. This NO production is associated with glaucomatous regions of the lung These data indicate that NO production is associated temporally and anatomically with silica-induced lung disease in a rat inhalation model [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Animals Fibrosis Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Inhalation-studies Laboratory-animals Lung-disorders Lung-function Lung-irritants Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Silicates

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023062
• Citation:
  FASEB J 2002 Mar; 16(5):A962
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f8791678383f564b343212186cca22a4d113b46c77c85b5b192b2c9dd89001df37691e09d102fb2043db2c5bbe9028637c57935cb7cdd138ee20750b401b92fe
• Download URL:
  https://stacks.cdc.gov/view/cdc/199646/cdc_199646_DS1.pdf
• File Type:
  [PDF - 123.42 KB ]