Antisense Inhibition of Translation Initiation Factor 3 Reverses Its Oncogenic Potential

Details

• Personal Author:
  Joseph P ; Lei YX ; Ong TM
• Description:
  Recently we have identified, cloned, and characterized the mouse Translation Initiation Factor 3 (TIF3, GenBank Accession Number AF 271072) as a novel cadmium-responsive proto-oncogene. Presently, additional studies regarding the oncogenic potential of TIF3 have been carried out. Transfection of NIH3T3 cells with the pcDNA3.1 expression vector containing the TIF3 cDNA in the sense (5'-->3') orientation resulted in overexpression of the encoded 36 kDa protein. Transfection-mediated overexpression of TIF3 protein resulted in transformation of the cells as evidenced from the appearance of transformed foci. Cotransfection of the cells with a mixture of plasmid DNA consisting of TIF3 cDNA in the sense and in the antisense orientation resulted in significant inhibition of translation of the TIF3 protein. Antisense (3'-->5') TIF3 mRNA-mediated inhibition of translation of TIF3 protein, furthermore, resulted in inhibition of TIF3-mediated transformation of NIH3T3 cells as evidenced from the decrease in the number of transformed foci. These results further confirm that overexpression of TIF3 is oncogenic and the antisense TIF3 mRNA expression reverses its oncogenic potential. [Description provided by NIOSH]
• Subjects:
  Cadmium Digestive System Digestive System Diseases Environmental Pollution Genetics Liver Occupational Exposure Occupational Health Respiratory Tract Diseases Safety Urogenital Diseases Urogenital System

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• Keywords:
  Antisense MRNA Author Keywords: Cadmium Cadmium-compounds Cell-transformation Cell Transformation Environmental-contamination Exposure-levels Gene Expression Genes Kidney-damage Liver-damage NIH3T3 Cells Occupational-exposure Occupational-hazards Oncogenesis Oncogenic-agents Respiratory-system-disorders TIF3 Translation Initiation Factor 3 Workers

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• ISSN:
  0270-3211
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  403-409
• Volume:
  22
• Issue:
  6
• NIOSHTIC Number:
  nn:20023018
• Citation:
  Teratog, Carcinog, Mutagen 2002 Nov; 22(6):403-409
• Contact Point Address:
  Tong-man Ong, MS 3014, Toxicology and Molecular Biology Branch, HELD, CDC/NIOSH, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  too2@cdc.gov
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9)
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Teratogenesis, Carcinogenesis, and Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3047e5add3b71cb93d7eacdb5ec777397ce3ee8c1e5bb7dc68995759b29a2c8ded81fe20a9281ea69fea90e07bc682fb9e887154590f81ded6e9c91259ea9c22
• Download URL:
  https://stacks.cdc.gov/view/cdc/199608/cdc_199608_DS1.pdf
• File Type:
  [PDF - 181.25 KB ]