IGF-I, IGF-II, and IGF-Receptor-1 Transcript and IGF-II Protein Expression in Myostatin Knockout Mice Tissues

Details

• Personal Author:
  Batelli L ; Gahr SA ; Hubbs, Ann F. ; Killefer J ; Kocamis H
• Description:
  Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were performed to demonstrate whether a correlation exists between insulin-like growth factors (IGFs)-positive regulators of growth-and myostatin, a negative regulator of muscle growth. IGF-I, -II, and IGF-receptor-1 (IGF-R1) mRNA and IGF-II protein expressions were determined in control and myostatin knockout mice tissues. IGF-I gene expressions were similar between control and knockout mice tissues, whereas IGF-II mRNA levels were significantly higher in myostatin knockout mice kidney and soleus muscles than those of control mice (P <.01). IGF-R1 mRNA levels from control mice heart (P <.05) and kidney (P <.01) were significantly higher than in myostatin knockout mice, whereas levels were lower in pectoralis muscle of control mice than knockout mice (P <.01). The strongly IGF-II-positive cells in soleus muscle were more common in myostatin knockout mice and were seen in a few foci in control mice. IGF-II immunoreactivity in both control and myostatin knockout mice kidneys was localized to the epithelium of renal tubules and collecting ducts. Reciprocal changes in the expression of myostatin and IGF-II and IGF-R1 may underlie normal growth of skeletal muscle and other organs in mammals, and the changes in these tissues associated with disease. [Description provided by NIOSH]
• Subjects:
  Animals Genetics Growth Laboratories Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal-studies Author Keywords: Immunohistochemistry Genes Growth-factors Insulin-like Growth Factor Insulin-like Growth Factor Receptors Kidneys Laboratory-animals Muscle-tissue Muscles Myostatin Knockout Mice Proteins RT-PCR

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• ISSN:
  0148-639X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  55-63
• Volume:
  26
• Issue:
  1
• NIOSHTIC Number:
  nn:20022980
• Citation:
  Muscle Nerve 2002 Jul; 26(1):55-63
• Contact Point Address:
  Division of Animal and Veterinary Sciences, P.O. Box 6108, West Virginia University, Morgantown, WV 26506-6108, USA
• Email:
  jkillef@wvu.edu
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Muscle & Nerve
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2d1b0f6806f2514841ac5c4bd134b465bc61b1bd9b27d5223a2ee5566988acf3e8db2815965452f079d3e8ac7d50a422945e712968bb0ffe4b39b8d622bf808c
• Download URL:
  https://stacks.cdc.gov/view/cdc/199580/cdc_199580_DS1.pdf
• File Type:
  [PDF - 15.70 MB ]