Determination of Alachlor and Its Metabolites in Rat Plasma and Urine by Liquid Chromatography – Electrospray Ionization Mass Spectrometry

Details

• Personal Author:
  DeHaven J ; Qiao G ; Yocum A ; Zang LY
• Description:
  A method based on liquid chromatography (LC) in combination with mass spectrometry (MS) for the analysis of alachlor (ALA) and its metabolites, 2-chloro-N-[2,6-diethylphenyl]acetamide (CDEPA) and 2,6-diethylaniline (DEA), in rat plasma and urine has been developed. 13C-labeled ALA was used as the internal standard for quantitation. The analyte in plasma or urine was isolated using a Waters Oasis HLB extraction plate. The mass spectrometer was operated in the ESI MS-SIM mode with a programming procedure. The retention times for ALA, CDEPA and DEA were 1.84, 3.11 and 4.12 min, respectively. The limits of quantification (LOQ) for ALA, CDEPA and DEA were 2.3, 0.8 and 0.8 ng per injection, respectively. The linear fit of analyte to mass response had an R2 of 0.99. Reproducibility of the sample handling and LC-MS analysis had a RSD of < or = 10%. The average recoveries for these analytes in rat plasma were better than 90%. Similar results were obtained with rat urine. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Energy Metabolism Laboratories Mass Spectrometry Occupational Health Safety Urine
• Keywords:
  Animal-studies Author Keywords: Alachlor In-vitro-study In-vivo-study Ionization Laboratory-animals Liver Mass-spectrometry Metabolites Urinalysis
• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  93-101
• Volume:
  767
• Issue:
  1
• NIOSHTIC Number:
  nn:20022917
• Citation:
  J Chromatogr B 2002 Feb; 767(1):93-101
• Contact Point Address:
  Lun-Yi Zang, Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, MS 3030, Morgantown, WV 26505-2888, USA
• Email:
  laz7@cdc.gov
• CAS Registry Number:
  Alachlor (CAS RN 15972-60-8)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:152c2f0faee936f02008f7838c9c6253d82bd87d973eddfacf698bd8a9cf97af3d6ad01d678edd4a90be76223d5484244936b1d495ae21e413d61f7c1ac78e70
• Download URL:
  https://stacks.cdc.gov/view/cdc/199531/cdc_199531_DS1.pdf
• File Type:
  [PDF - 264.67 KB ]