Epithelium-Dependent Relaxation Responses of Guinea-Pig Isolated, Perfused Trachea to Hypertonic Solution Involves Carbon Monoxide (CO)

Details

• Personal Author:
  Dowdy J ; Fedan, Jeffery S. ; Johnston RA ; Reasor MJ ; Van Scott MR
• Description:
  Application of hypertonic solution to the mucosal surface of the guinea-pig isolated, perfused trachea (IPT) elicits epithelium-dependent relaxation of airway smooth muscle via epithelium-derived relaxing factor (EpDRF), the identity of which is unknown. The purpose of this study was to examine whether CO mediates smooth muscle relaxation. After contracting the smooth muscle with serosally-applied methacoline (3 x 10-7 M), the PT was challenged with mucosally-administered D-mannitol (D-M; 120-160 mosM), a non-permeant osmolyte. The effects of inhibitors on the response to D-M were examined. Neither cyclooxygenase inhibition with indomethacin (3x10-6 M; 30 min) nor injibition of nitric oxide (NO) synthase with Nw-nitro-L-arginine methyl ester (L-NAME; 10-4 M; 30 min) affected the response to D-M, indicating that neither prostanoids nor NO are involved. Upon addition of the CO scavenger, hemoglobin (Hb; 10-6M; 30 min) the IPT developed small contractile responses. In the presence of Hb the relaxation to D-M was inhibited significantly. Neither Hb nor ZnPP abolished completely the relaxation response to D-M. These results suggest that epithelium-derived CO participated in the hypertonicity-induced relaxation of the trachea. [Description provided by NIOSH]
• Subjects:
  Lung Musculoskeletal System Occupational Health Respiratory System Safety
• Keywords:
  Cell-function Cellular-reactions In-vitro-studies Muscle-cells Muscle-function Muscles Pulmonary-system
• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Proceedings ; Abstract
• Place as Subject:
  Massachusetts ; North Carolina ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  165
• Issue:
  8
• NIOSHTIC Number:
  nn:20022849
• Citation:
  Am J Respir Crit Care Med 2002 Apr; 165(8)(Suppl):A65
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine, 2002 International Conference, The American Thoracic Society, Atlanta Georgia, May 17-22, 2002
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a250be50d8c433b8c435e357436305ee3d152680b643fb2bf0c3fd043fdf6d2be45f1dabbca4ce4b92e2da5897a3d5f1f9d280939a097898b6ab7179a571ad73
• Download URL:
  https://stacks.cdc.gov/view/cdc/199480/cdc_199480_DS1.pdf
• File Type:
  [PDF - 344.86 KB ]