Enhanced Nitric Oxide and Reactive Oxygen Species Production and Damage After Inhalation of Silica

Details

• Personal Author:
  Castranova, Vincent ; Hubbs A ; Khan A ; Landsittel D ; McLaurin J ; Millecchia L ; Pack D ; Porter DW ; Ramsey D ; Robinson VA ; Teass A ; Willard P
• Description:
  In previous reports from this study, measurements of pulmonary inflammation, bronchoalveolar lavage cell cytokine production and nuclear factor-B activation, cytotoxic damage, and fibrosis were detailed. In this study, we investigated the temporal relationship between silica inhalation, nitric oxide (NO), and reactive oxygen species (ROS) production, and damage mediated by these radicals in the rat. Rats were exposed to a silica aerosol (15 mg/m3 silica, 6 h/day, 5 days/wk) for 116 days. We report time-dependent changes in 1) activation of alveolar macrophages and concomitant production of NO and ROS, 2) immunohistochemical localization of inducible NO synthase and the NO-induced damage product nitrotyrosine, 3) bronchoalveolar lavage fluid NOx and superoxide dismutase concentrations, and 4) lung lipid peroxidation levels. The major observations made in this study are as follows: 1) NO and ROS production and resultant damage increased during silica exposure, and 2) the sites of inducible NO synthase activation and NO-mediated damage are associated anatomically with pathological lesions in the lungs. [Description provided by NIOSH]
• Subjects:
  Aerosols Aluminum Silicates Animals Cytotoxins Environmental Monitoring Fibrosis Laboratories Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide Silicosis

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• Keywords:
  Animal-studies Author Keywords: Silicosis Cytotoxicity Exposure-assessment Exposure-levels Inhalation-studies Laboratory-animals Lung-burden Nitrotyrosine Oxidant Injury Oxidation Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Silica-dusts Silicates

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• ISSN:
  1040-0605
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  283
• Issue:
  2
• NIOSHTIC Number:
  nn:20022831
• Citation:
  Am J Physiol Lung Cell Mol Physiol 2002 Aug; 283(2):L485-L493
• Contact Point Address:
  D.W. Porter, Pathology and Physiology Research Branch, Health Effects Laboratory Div., National Institute for Occupational Safety and Health, 1095 Willowdale Rd., M/S 2015, Morgantown, WV 26505
• Email:
  DPorter@cdc.gov
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8f0799f08b8eb6a58aeb28cebb3a19ecab57eac113fb2ef21b1b0bc997708a6fe0e0bb5e14ca3cf9b6c8a7a0cbf54f6aca8cea92510098fe32c59bdb50ec3ee9
• Download URL:
  https://stacks.cdc.gov/view/cdc/199468/cdc_199468_DS1.pdf
• File Type:
  [PDF - 545.18 KB ]