Biomarker Characterization of Asphalt Fume Exposure

Details

• Personal Author:
  Frazer DG ; Goldsmith T ; Law B ; Lewis, Daniel M. ; Mosley A ; Siegel, Paul D. ; Simpson J ; Stone S ; Wang JJ
• Description:
  Each year more than 60 million tones of asphalt are produced and used worldwide. It has been estimated that approximately two million workers were employed and potentially exposed to asphalt. Health concern from exposure to asphalt is related to its content of hazardous chemicals, such as polycyclic aromatic hydrocarbons (PAHs). The development of a biomarker to provide an assessment of the integrated external exposure following uptake by living system through inhalation is valuable for this kind of complex mixture exposure. Many PAHs are metabolized to hydroxyl-PAHs and excreted in urine; therefore, urinary hydroxyl-PAH was used as a biomarker for asphalt fume exposure in this study. Female Sprague-Dawley rats and B6C3F1 mice were exposed to asphalt fume in a whole body inhalation chamber for 5 or 10 days (3.5 hrs/day, 71-97 mg/m 3 ). Clean air chamber animals were used as controls. The test asphalt was the type used by paving industry throughout the Midwestern United States. The fume was generated under simulated road paving conditions. Urine sample preparation for the analysis of hydroxyl-PAHs metabolites included enzymatic digestion, solid phase extraction, and isotope dilution GC/MS and microflow LC-Q-TOF MS detections. Four priorities PAH metabolites, including, 1-hydroxypyrene, 9-phenanthrenol, 1-chrysenol, and 2-naphthalenol were identified and determined in urine. Isotope-dilution has proved to be useful for quantification of those metabolites. Preliminary results indicated that urinary PAH metabolite could be a sensitive biomarker following asphalt fume exposure. The method developed in this study has potential application to monitor of PAHs from occupational mixture exposure. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Chemistry, Analytic Hydrocarbons Inhalation Exposure Laboratories Occupational Health Safety Urine
• Keywords:
  Analytical-chemistry Animal-studies Asphalt-fumes Exposure-levels Humans Inhalants Laboratory-animals Urinalysis Urine-chemistry Workers
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  66
• NIOSHTIC Number:
  nn:20022816
• Citation:
  Toxicologist 2002 Mar; 66(1-S):284
• CAS Registry Number:
  1-Hydroxypyrene (CAS RN 5315-79-7) ; 2-Naphthol (CAS RN 135-19-3)
• Federal Fiscal Year:
  2002
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6e162bc12b5759e4a2006d8436e07476e68b4260d30de7e5038421fa3eac344a25f69ab28332dbdbb2d397bc9e67f16873fcf5608bab23b20bc1a7e32068c807
• Download URL:
  https://stacks.cdc.gov/view/cdc/199455/cdc_199455_DS1.pdf
• File Type:
  [PDF - 195.30 KB ]