Evidence for Lipopolysaccharide-Induced Differentiation of RAW264.7 Murine Macrophage Cell Line into Dendritic Like Cells

Details

• Personal Author:
  Lewis, Daniel M. ; Saxena RK ; Vallyathan V
• Description:
  Effect of lipopolysaccharide (LPS) on RAW 264.7 macrophage cell line was studied. LPS-treated RAW264 7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mg/ml), almost 70% RAW 264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell acti vation (B 7.1, B 7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW 264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells. [Description provided by NIOSH]
• Subjects:
  Antigens Musculoskeletal System Neoplasms Occupational Health Safety
• Keywords:
  Author Keywords: Dendritic Cells Bone-marrow Cancer Differentiation Flow Cytometry In-vitro-study Leukocytes Lipopolysaccharide Morphology Myeloid-tissue
• ISSN:
  0250-5991
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  129-134
• Volume:
  28
• Issue:
  1
• NIOSHTIC Number:
  nn:20022595
• Citation:
  J Biosci 2003 Feb; 28(1):129-134
• Contact Point Address:
  School of Life Sciences, Jawaharlal Nehru University, New Delhi 110 067, India
• Email:
  rksaxena@mail.jnu.ac.in
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biosciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:71b40c0e16c0d59738cf8c70f15d34995d37ce557f500fa1bd25ac0d988947e5431be1b34c5b07d60b6894250445c47503831ff05e6e4fae9cea9191fbeb1663
• Download URL:
  https://stacks.cdc.gov/view/cdc/199323/cdc_199323_DS1.pdf
• File Type:
  [PDF - 366.02 KB ]