Role of Metal-Induced Reactive Oxygen Species Generation in Lung Responses Caused by Residual Oil Fly Ash

Details

• Personal Author:
  Antonini JM ; Leonard, Stephen S. ; Lewis AB ; Roberts, Jennifer R. ; Shi X ; Taylor MD
• Description:
  Inhalation of residual oil fly ash (ROFA) increases pulmonary morbidity in exposed workers. We examined the role of reactive oxygen species (ROS) in ROFA-induced lung injury. ROFA was collected from a precipitator at Boston Edison Co., Everett, MA, USA. ROFA (ROFA-total) was suspended in saline, incubated for 24 h at 37 C, centrifuged, and separated into its soluble (ROFA-sol.) and insoluble (ROFA-insol.) fractions. Sprague-Dawley rats were intratracheally instilled with saline or ROFA-total or ROFA-sol. or ROFA-insol. (1 mg/100 g body wt.). Lung tissue and bronchoalveolar lavage cells were harvested at 4, 24, and 72 h after instillation. Chemiluminescence (CL) of recovered cells was measured as an index of ROS production, and tissue-lipid-peroxidation was assessed to determine oxidative injury. Significant amounts of Al, Fe, and Ni were present in ROFA-sol., whereas ROFA-insol. contained Fe, V, and Al. Using electron spin resonance (ESR), significantly more hydroxyl radicals were measured in ROFA-sol. as compared to ROFA-insol. None of the ROFA samples had an effect on CL or lipid peroxidation at 4 h. Treatment with ROFA-total and ROFA-insol. caused significant increases in both CL (at 24 h) and lipid peroxidation (at 24 and 72 h) when compared to saline control value. ROFA-sol. significantly reduced CL production at 72 h after treatment and had no effect on lipid peroxidation at any time point. In summary, ROFA, particularly its soluble fraction, generated a metal-dependent hydroxyl radical as measured by a cell-free ESR assay. However, cellular oxidant production and tissue injury were observed mostly with the ROFA-total and ROFA-insol. particulate forms. ROS generated by ROFA-sol. as measured by ESR appear not to play a major role in the lung injury caused after ROFA exposure. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Air Pollution Air Pollution, Indoor Animals Dust Occupational Health Particulate Matter Respiratory Tract Diseases Safety
• Keywords:
  Air-contamination Air-quality Animal-studies Author Keywords: Electron Spin Resonance Combustion-products Fly-ash In-vitro-study Lung Particulate-dust Particulates Pulmonary-system-disorders Reactive Oxygen Species Residual Oil Fly Ash Respirable-dust Respiratory-system-disorders

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• ISSN:
  0250-5991
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  13-18
• Volume:
  28
• Issue:
  1
• NIOSHTIC Number:
  nn:20022582
• Citation:
  J Biosci 2003 Feb; 28(1):13-18
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Work Environment and Workforce: Mixed Exposures
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biosciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3047c50f341a7e71da9412ef7b51e01890984a7bdc1e3439a40e38ac19cf4473b5c79da1782b307720ccb2b579107dc97ae7251401c2608f1ab1316f8faced1b
• Download URL:
  https://stacks.cdc.gov/view/cdc/199314/cdc_199314_DS1.pdf
• File Type:
  [PDF - 209.56 KB ]