Oxidative DNA Damage Is Associated with Intense Noise Exposure in the Rat

Details

• Personal Author:
  Franks J ; Mathias P ; Murphy W ; Toraason M ; Van Campen, Luann E.
• Description:
  Increasing evidence suggests that noise-induced hearing loss may be reduced or prevented with antioxidant therapy. Biochemical markers of reactive oxygen species (ROS)-induced damage can help elucidate possible treatment timing constraints. This study examined the time course of ROS damage following a 2-h, broad-band noise exposure resulting in permanent threshold shift in 35 Long-Evans rats. Cochlea, brain, liver, serum and urine were analyzed at 1, 3, 8, 72, and 672 h (28 days) after exposure. Oxidative DNA damage was assessed by measuring 8-hydroxy-2'-deoxyguanosine (8OHdG) by high performance liquid chromatography with electrochemical detection. Lipid peroxidation was measured via the thiobarbituric acid-reactive substances (TBARS) colorimetric assay for detection of aldehydes (e.g., malondialdehyde). Auditory brainstem response and distortion product otoacoustic emission thresholds showed progressive elevation for the 3- and 8-h groups, then notable recovery for the 72-h group, and some worsening for the 672-h group. 8OHdG was significantly elevated in cochlea in the 8-h group, and in brain and liver for the 72-h group. TBARS were significantly elevated in serum for the 72-h group. Based upon oxidative DNA damage present in cochlea following intense noise, we postulate that the first 8 h following exposure might be a critical period for antioxidant treatment. [Description provided by NIOSH]
• Subjects:
  Aldehydes Animals Antioxidants DNA Formaldehyde Hearing Loss, Noise-Induced Liver Nervous System Nervous System Diseases Noise Occupational Health Safety

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• Keywords:
  8-Hydroxy-2P-deoxyguanosine Animal-studies Auditory-system Author Keywords: Free Radical Brain-disorders Cochlea DNA-damage Lipid-peroxidation Liver-disorders Noise-exposure Noise-induced-hearing-loss Oxidative Stress Reactive Oxygen Species Thiobarbituric Acid-reactive Substance

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• ISSN:
  0378-5955
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  29-38
• Volume:
  164
• Issue:
  1
• NIOSHTIC Number:
  nn:20022398
• Citation:
  Hear Res 2002 Feb; 164(1-2):29-38
• Contact Point Address:
  Engineering and Physical Hazards Branch, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, Cincinnati, OH 45226, USA
• Email:
  vancampen_1_e@lilly.com
• CAS Registry Number:
  Benzyl alcohol (CAS RN 100-51-6) ; Toluene (CAS RN 108-88-3)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Hearing Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:33b0154349f4484896a39c2a32239159cb179affcc2249d4afa4a8d947526617d27b1bfb790bb5f4eb029fb48fcf864fd7a6a1a3196f79ec9f84283f20c3b01b
• Download URL:
  https://stacks.cdc.gov/view/cdc/199190/cdc_199190_DS1.pdf
• File Type:
  [PDF - 360.67 KB ]