Differential Role of Hydrogen Peroxide in UV-Induced Signal Transduction

Details

• Personal Author:
  Castranova, Vincent ; Costa M ; Ding M ; Huang C ; Leonard, Stephen S. ; Li J ; Shi X ; Vallyathan V
• Description:
  The present study investigated the differential requirement of ROS in UV-induced activation of these pathways. Exposure of the mouse epidermal C141 cells to UV radiation led to generation of ROS as measured by electron spin resonance (ESR) and by H2O2 and O2. fluorescence staining assay. Treatment of cells with UV radiation or H2O2 also markedly activated Erks, JNKs, p38 kinase and led to increases in phosphorylation of Akt and p70(S6k) in mouse epidermal JB6 cells. The scavenging of UV-generated H2O2 by N-acety-L-cyteine (NAC, a general antioxidant) or catalase (a specific H2O2 inhibitor) inhibited UV-induced activation of JNKs, p38 kinase, Akt and p70(S6k), while it did not show any inhibitory effects on Erks activation. Further, pretreatment of cells with sodium formate (an .OH radical scavenger) or superoxide dismutase (O2-. radical scavenger) did not inhibit any of these pathways. These results demonstrate that H2O2 generation is required for UV-induced phosphorylation of Akt and p70(S6k), and involved in activation of JNKs and p38 kinase, but not Erks [Description provided by NIOSH]
• Subjects:
  Animals Antioxidants Free Radicals Genetics Neoplasms Occupational Health Radiation Safety Skin Skin Diseases Ultraviolet Rays

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• Keywords:
  Animal-studies Author Keywords: Oxidative Stress Cancer Cell-alteration Cell-biology Cell-differentiation Free-radicals Genes MAP Kinases Molecular-biology Oxidative-phosphorylation Oxidative-processes Oxyradical Scavengers Protein-biochemistry Radiation-effects Skin-cancer Ultraviolet-radiation

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• ISSN:
  0300-8177
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  81-90
• Volume:
  234
• Issue:
  1
• NIOSHTIC Number:
  nn:20022163
• Citation:
  Mol Cell Biochem 2002 May-Jun; 234-235(1-2):81-90
• Contact Point Address:
  Nelson Institute of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, NY 10987, USA
• Email:
  chuanshu@env.med.nyu.edu
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Molecular and Cellular Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f23da43e65c8ee7f0f76fcedaf075e57b559704ea71c7c667f491a95af61fbb97ebcb87e78624598e1479f70a4bdb2307b29835dda477d4a354f0aa7080fe4bd
• Download URL:
  https://stacks.cdc.gov/view/cdc/199048/cdc_199048_DS1.pdf
• File Type:
  [PDF - 490.62 KB ]