Second Inter-Laboratory Study Comparing Endotoxin Assay Results from Cotton Dust

Details

• Personal Author:
  Bartlett K ; Chew V ; Chun DT ; Gordon T ; Gunn VC ; Jacobs R ; Larsson BM ; Lewis, Daniel M. ; Liesivuori J ; Michel O ; Rylander R ; Thorne PS ; White EM ; Wurtz H
• Description:
  Previously, a large two-part inter-laboratory round robin endotoxin assay study was completed. This first study showed that when cotton dust samples, which are practically identical, are assayed for endotoxin that the intra- laboratory results had a very small variation while intra-laboratory results of the sample had a very high variation. In the first part of the study, each laboratory followed its own in-house assay protocol; but in the second part of the study, when the extraction protocol was standardized, the inter-laboratory results showed a lower variation, which suggested that with further standardization, further reduction of differences between laboratories might be achieved in order that results between laboratories would become more comparable. The results stimulated interest in extending the study to include cotton dust with two levels of endotoxin, standardization of the extraction protocol, and using the same assay kit from the same production lot. The results of this second round robin endotoxin assay study indicate that differences between laboratories are still high, but most of the laboratories could discern the cotton dusts with the different levels of endotoxin [Description provided by NIOSH]
• Subjects:
  Dust Endotoxins Laboratories Occupational Health Quality Control Safety
• Keywords:
  Author Keywords: Endotoxin Assay Cotton-dust Cotton Dust Exposure-limits Inter-laboratory Endotoxin Assay Study Interlaboratory Endotoxin Assay Study Laboratory-testing Limulus Amoebocyte Lysate Test (LAL) Lipopolysaccharides (LPS) Quality-control Quality-standards Round Robin Endotoxin Assay Study Sampling Sampling-methods Standards Testing-equipment

  More +
• ISSN:
  1232-1966
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  49-53
• Volume:
  9
• Issue:
  1
• NIOSHTIC Number:
  nn:20022162
• Citation:
  Ann Agric Environ Med 2002 Jan; 9(1):49-53
• Contact Point Address:
  Cotton Quality Research Station, Agricultural Research Service, US Department of Agriculture, P.O. Box 792, Clemson, SC 29633, USA
• Email:
  dtwchun@mindspring.com
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  True
• Source Full Name:
  Annals of Agricultural and Environmental Medicine
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:27ef85f3e40f29aaef8084587795635c6b922f848ade80932466eadfe6be53dca42995c54ecc666a043294a2a9dd516ffa8663667b7812b1d6996372eb5e3500
• Download URL:
  https://stacks.cdc.gov/view/cdc/199047/cdc_199047_DS1.pdf
• File Type:
  [PDF - 35.32 KB ]