Role of Intrinsic Airway Neurons in Ozone-Induced Airway Hyperresponsiveness in Ferret Trachea

Details

• Personal Author:
  Dey RD ; Fedan, Jeffery S. ; Frazer DG ; Maize DF Jr. ; Satterfield BE ; Wu Z-X
• Description:
  Exposure to ozone (O(3)) enhances airway responsiveness, which is mediated partly by the release of substance P (SP) from airway neurons. In this study, the role of intrinsic airway neurons in O(3)-induced airway responses was examined. Ferrets were exposed to 2 ppm O(3) or air for 1 h. Reactivity of isolated tracheal smooth muscle to cholinergic agonists was significantly increased after O(3) exposure, as were contractions to electrical field stimulation at 10 Hz. Pretreatment with CP-99994, a neurokinin type 1 receptor antagonist, partially abolished the O(3)-induced reactivity to cholinergic agonists and electrical field stimulation. The O(3)-enhanced airway responses were present in tracheal segments cultured for 24 h, a procedure shown to deplete sensory nerves while maintaining viability of intrinsic airway neurons, and all the enhanced smooth muscle responses were also diminished by CP-99994. Immunocytochemistry showed that the percentage of SP-containing neurons in longitudinal trunk and the percentage of neurons innervated by SP-positive nerve fibers in superficial muscular plexus were significantly increased at 1 h after exposure to O(3). These results suggest that enhanced SP levels in airway ganglia contribute to O(3)-induced airway hyperresponsiveness. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Irritants Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Animal-studies Exposure-assessment Exposure-levels Laboratory-animals Pulmonary-function Respiratory-irritants
• ISSN:
  8750-7587
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  371-378
• Volume:
  91
• Issue:
  1
• NIOSHTIC Number:
  nn:20021931
• Citation:
  J Appl Physiol 2001 Jul; 91(1):371-378
• Contact Point Address:
  R. D. Dey, Dept. of Anatomy, PO Box 9128, West Virginia University, Morgantown, WV 26506
• Email:
  rdey@hsc.wvu.edu
• CAS Registry Number:
  Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Applied Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:af454b7ec95d02d7849d84b3cd191f2c507141873e1a9f81f78752c6b0bc3e635059fd3ca3e252568f87c76b3d1ce3a5c34173f73382ffb07f1bdab2c29c71c6
• Download URL:
  https://stacks.cdc.gov/view/cdc/199006/cdc_199006_DS1.pdf
• File Type:
  [PDF - 335.61 KB ]