Cytokine and Nitric Oxide Release by J774A.1 Macrophages Is Not Regulated by Estradiol

Details

• Personal Author:
  Huffman LJ ; Schuller KC ; Woodfork KA
• Description:
  Estradiol is able to regulate the release of inflammatory mediators by macrophages; however, the presence, extent, and direction of this modulation varies with species, tissue of origin, and cell culture conditions. This study examines the effects of 17-beta-estradiol (E2) on the release of inflammatory mediators by the J774A.1 mouse macrophage cell line. For experiments, cells were plated in phenol red-free DMEM containing 5% charcoal-dextran stripped calf serum. Western analysis showed that J774A.1 cells contain the estrogen receptor alpha (ER alpha) protein. We found that physiological and pharmacological levels of E2 (10(-12) M-10(-6) M) have no effect on the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), or monocyte chemoattractant protein-1 (MCP-1). This suggests that J774A.1 cells grown under these culture conditions would be useful for the investigation of non-estrogen-dependent mechanisms by which certain endocrine disruptors may affect their targets in macrophages. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Endocrine System Nitric Oxide Occupational Health Safety
• Keywords:
  Author Keywords: Macrophage Cell-biology Cell-cultures Cell-function Cellular-function Cellular-reactions Endocrine-system Endocrine Disruptors Estrogen In-vitro-study Interleukin-6 Monocyte Chemoattractant Protein-1 Nitric Oxide Oxides Physiological-effects Tumor Necrosis Factor-a

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• ISSN:
  0024-3205
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  69
• Issue:
  19
• NIOSHTIC Number:
  nn:20021930
• Citation:
  Life Sci 2001 Sep; 69(19):2287-2294
• Contact Point Address:
  Karen A. Woodfork, Department of Biochemistry and Molecular Pharmacology, Robert C. Byrd Health Sciences Center, West Virginia University, P.O. Box 9142, Morgantown, WV 26506
• Email:
  kwoodfork@hsc.wvu.edu
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9a3c1556eeaba6e25a5e1dc608675fb698a673306a63ec239ada29cb243280e716085081b2dfb5d4312abcf4ecfa8fb7b8508d2812e5c4ade36c86bedf597760
• Download URL:
  https://stacks.cdc.gov/view/cdc/199005/cdc_199005_DS1.pdf
• File Type:
  [PDF - 602.15 KB ]