The Role of NFkB in (1–>3)-B-Glucan (Zymosan a) Induced TNF-Alpha Production

Details

• Personal Author:
  Castranova, Vincent ; Frazer DG ; Shi X ; Ye J ; Young S
• Description:
  The signal transduction pathway in the inflammatory response caused by a fungal cell wall component (1-->3)-B-glucan is not well understood. The present study used zymosan A-induced tumor necrosis factor (TNF)-alpha production as a model to explore the signal transduction pathway for B-glucan stimulation in RAW264.7 cells. Zymosan A increased TNF-alpha production in RAW264.7 cells in a time and concentration dependent pattern with the optimal time and concentration occurring at 23 hr and 100 ug/mL zymosan A, respectively. A gel shift assay was used to examine the DNA-binding activity of NFkB in the zymosan A- treated cells. This NFkB activity was enhanced by this stimulant. NFkB activation was associated with TNF-alpha production, since the cells pre-treated with a known NFkB inhibitor (caffeic acid phenethyl ester) decreased both NFkB activation and TNF-alpha production. The dependence of TNF-alpha transcriptional initiation on kB sites was investigated using a luciferase reporter assay. Both wild type and kB-mutated type TNF-alpha promoters were utilized to study the dependence of TNF-alpha promoter on kB sites. The results demonstrated that the activation of TNF-alpha promoter was dependent on the activation of NFkB. A NFkB specific reporter was used in conjunction with TNF-alpha wild type reporter to evaluate the temporal relationship between the NFkB activation and induction of TNF-alpha reporter activity. The results showed that the peak of NFkB activation occurred at 5 hrs which proceeded the peak of TNF-alpha promoter activation (30 hrs). The above results suggest that activation of NFkB is one of the pathways involved in zymosan A-induced TNF-alpha production. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Fungi Fungicide, Industrial Occupational Health Safety
• Keywords:
  Cell Cultures Fungal Infections Fungicides Models Stimulants
• Publisher:
  The National Cotton Council
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  15
• NIOSHTIC Number:
  nn:20021101
• Citation:
  Proceedings of the 24th Cotton and Other Organic Dusts Research Conference, January 7-8, 2000, San Antonio, TX, Wakelyn-PJ, Jacobs-RR, Rylander-R, eds., Memphis, TN: The National Cotton Council, 2000 Jan; :15
• Editor(s):
  Wakelyn PJ; Jacobs RR; Rylander R
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the 24th Cotton and Other Organic Dusts Research Conference
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0984d6b03680b322193ad8e0eaba894b5b37099d1a18c36192b1d817cbdac8bb0099c8f8c650a5272b99036c8fe5cf9c856195c0e9ac6f72f79b2e59a49ae42d
• Download URL:
  https://stacks.cdc.gov/view/cdc/198649/cdc_198649_DS1.pdf
• File Type:
  [PDF - 232.12 KB ]