Effects of Asphalt Fume Condensate (AFC) Exposure on the Pulmonary Cytochrome P450 (CYP450) System

Details

• Personal Author:
  Barger M ; Castranova, Vincent ; Kreich AJ ; Ma JYC ; Yang HM
• Description:
  Exposure to asphalt fumes is a concern due to the presence of polycyclic aromatic hydrocarbons (PAH) in asphalt. Bioactivation of many PAH requires metabolism by the CYP450 system. The objective of this study was to evaluate the effects of exposure of rats to AFC on the pulmonary CYP450 system. Rats were intratracheally instilled with saline, 0.1, 0.5, or 2 mg AFC for 3 consecutive days and sacrificed the following day. Total microsomal protein and CYP2Bl and CYP1A1 isozyme levels were determined. The activities associated with CYP2B1 and CYP1A1 were measured by the O-dealkylation of 7-pentoxyresorufin (7-PR) and 7-ethoxyresorufin (7-ER), respectively. AFC exposure did not cause significant changes in total microsomal protein content. CYP2B1 and CYP1A1 levels in AFC-exposed lungs were not significantly different from control as determined by Western blot analysis. However, microsomes from AFC-exposed rats exhibited a concentration- dependent increase in the activity of 7-PR and 7-ER O-dealkylase. These results demonstrate that AFC exposure did not alter total microsomal protein or CYP1A1 and CYP2B1 levels in the lung. However, AFC increased CYP2B1 and CYP1A1 activities in lung microsomes, suggesting that AFC exposure may significantly alter metabolism of PAH by the CYP450 system in the lung. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Animals Environmental Exposure Hydrocarbons Laboratories Lung Lung Diseases Occupational Health Polycyclic Aromatic Hydrocarbons Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Animal Studies Asphalt Fumes Bioactivation Exposure Levels Fumes Laboratory Animals Lung Disorders Polycyclic Aromatic Hydrocarbons Pulmonary System Disorders Respiratory System Disorders
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Indiana ; OSHA Region 3 ; OSHA Region 5 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  4
• NIOSHTIC Number:
  nn:20020995
• Citation:
  FASEB J 2000 Mar; 14(4):A173
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:046ea4385d4957d93b150df83bb6f5562ad437820ecd7ad7f0569c5b8bce921b2310775c2b434a465b23f276254bc38f4436cd2fe82c9f3908420d376f5b69ca
• Download URL:
  https://stacks.cdc.gov/view/cdc/198595/cdc_198595_DS1.pdf
• File Type:
  [PDF - 419.54 KB ]