Respirable Quartz and Kaolin Induction of Apoptosis in Rat Pulmonary Macrophage NR8383 Cells

Details

• Personal Author:
  Gao N ; Keane M ; Ong T ; Wallace WE
• Description:
  Respirable-sized quartz and kaolin dusts were tested for their induction of apoptosis in the rat pulmonary macrophage NR 8383 cell line. The effects of dust pre-incubation with the dipalmitoylphosphatidylcholine (DPPC) component of pulmonary surfactant on the induction of apoptosis were measured. Occupational exposure to respirable silica quartz dust can result in an acute inflammatory response followed by chronic fibrotic change in the lungs. Kaolin aluminosilicate dust generally is not associated with significant fibrotic lung disease. Pretreatment of dust particles with DPPC is known to suppress some otherwise prompt in vitro cytotoxic activities of quartz and kaolin. Apoptosis was measured by two assays: a propidium iodide staining procedure for DNA ploidy analysis using flow cytometry; and an ELISA assay for mono- and oligonucleosomes. Cells challenged in vitro for 6 hours by untreated quartz dust showed a dosedependent increase in apoptosis as indicated by both flow cytometry and ELISA assays. Kaolin was less strongly active than quartz in the ELISA assay and kaolin was active only at high concentrations in the flow cytometry assay. Cells also were challenged over a one- to five-day period by quartz or kaolin at a single concentration. DPPC pretreatment of both dusts delayed their induction of apoptosis as measured by the ELISA assay; partial activity was restored at 3 and 5 days after challenge. Neither DPPC-treated dust showed significant activity in the flow cytometry assay. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Animals Dust Laboratories Lung Diseases Occupational Exposure Occupational Health Particulate Matter Quartz Respiratory Tract Diseases Safety Silicon Dioxide

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• Keywords:
  Animal Studies Dust Particles Dusts In Vitro Studies Laboratory Animals Lung Disease Occupational Exposure Particulate Dust Particulates Pulmonary System Disorders Quartz Dust Respirable Dust Respiratory System Disorders Silica Dusts Silicates

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• ISSN:
  0893-6692
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  25
• Volume:
  35
• NIOSHTIC Number:
  nn:20020935
• Citation:
  Environ Mol Mutagen 2000 Jan; 35(Suppl 31):25
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Environmental and Molecular Mutagenesis
• Supplement:
  31
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1f8eb9fe2a827a41dcea4c76809cc7e2f3f0799931072527dceae62e02006e69f5e72eb7db52b7d1a52b43f18baeb8eb2fe302cc686b3a0ab438a29e2b97f997
• Download URL:
  https://stacks.cdc.gov/view/cdc/198560/cdc_198560_DS1.pdf
• File Type:
  [PDF - 221.95 KB ]