Requirement for DARPP-32 in Progesterone-Facilitated Sexual Receptivity in Female Rats and Mice

Details

• Personal Author:
  Allen PB ; Bibb J ; Dash PK ; Feinberg AA ; Greengard P ; Mani SK ; Mitchell AJ ; Moore AN ; O'Callaghan JP ; O'Malley BW ; Snyder GL
• Description:
  DARPP-32, a dopamine- and adenosine 3',5'-monophosphate (cAMP)-regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)-facilitated sexual receptivity in female rats and mice. The facilitative effect of P on sexual receptivity in female rats was blocked by antisense oligonucleotides to DARPP-32. Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. These increases were not inhibited by a D1 subclass dopamine receptor antagonist. P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice. [Description provided by NIOSH]
• Subjects:
  Animals Genetics Laboratories Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Animal Studies Gene Mutation Genes Genetic Factors Laboratory Animals Reproductive Effects Reproductive System Disorders
• ISSN:
  0036-8075
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; New York ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 6 ; OSHA Region 9 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  287
• Issue:
  5455
• NIOSHTIC Number:
  nn:20020746
• Citation:
  Science 2000 Feb; 287(5455):1053-1056
• Email:
  smani@bcm.tmc.edu
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5a48a779a74abcefec3268ecd82d9f55318be96659cc43ec609ade4a58545f14ae40018e8661f6b489e7994b3b23bbd4d8a3b2f3d2c8375f9b70bf7c9b093b74
• Download URL:
  https://stacks.cdc.gov/view/cdc/198416/cdc_198416_DS1.pdf
• File Type:
  [PDF - 179.08 KB ]