Impaired Cutaneous Wound Healing in Interleukin-6-Deficient and Immunosuppressed Mice

Details

• Personal Author:
  Gallucci RM ; Guriel JL ; Kommineni C ; Luster MI ; Matheson JM ; Simeonova PP ; Sugawara T
• Description:
  It has been postulated that an inflammatory response after cutaneous wounding is a prerequisite for healing, and inflammatory cytokines, such as interleukin-6 (IL-6), might be intimately involved in this process. IL-6-deficient transgenic mice (IL-6 KO) displayed significantly delayed cutaneous wound healing compared with wild-type control animals, requiring up to threefold longer to heal. This was characterized by minimal epithelial bridge formation, decreased inflammation, and granulation tissue formation. Using electrophoretic mobility shift assays of wound tissue from IL-6 KO mice, decreased AP-1 transcription factor activation was shown compared with wild-type mice 16 h after wounding. In situ hybridization of wound tissue from wild-type mice revealed IL-6 mRNA expression primarily in the epidermis at the leading edge of the wound. Delayed wound healing in IL-6 KO mice was reversed with a single dose of recombinant murine IL-6 or intradermal injection of an expression plasmid containing the full-length murine IL-6 cDNA. Treatment with rmIL-6 also reconstituted wound healing in dexamethasone-treated immunosuppressed mice. The results of this study may indicate a potential use for IL-6 therapeutically where cutaneous wound healing is impaired. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Occupational Health Safety
• Keywords:
  Animal Studies Author Keywords: Inflammation Cytokines Gene Therapy Health Care Injuries Laboratory Animals Occupational Health
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  15
• NIOSHTIC Number:
  nn:20020676
• Citation:
  FASEB J 2000 Dec; 14(15):2525-2531
• Contact Point Address:
  Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health MS 3014, 1095 Willowdale Rd., Morgantown, WV 26505, USA
• Email:
  myl6@cdc.gov
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  True
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3742ad0700b271f8ac6b9142e1bf1e3f5520bc456478e87150ae793ae8b28832d703ecc058c212e3a9c389fb36da9dd08eab98f9b915f67de439c7fe55de7c7e
• Download URL:
  https://stacks.cdc.gov/view/cdc/198357/cdc_198357_DS1.pdf
• File Type:
  [PDF - 235.67 KB ]