Effects of Various Treatments on the Quantitative Recovery of Endotoxin from Water-Soluble Metalworking Fluids

Details

• Personal Author:
  Brown, Mary E. ; Feng, H. Amy ; White EM
• Description:
  Three extraction methods were compared for their effectiveness in the quantitative removal of endotoxin from unused and used bulk water-soluble metalworking fluid (MWF) samples. Soluble, synthetic, and semisynthetic fluids were studied. The three modes of extraction consisted of (1) pyrogen-free water (PFW); (2) PFW and Tween 20 (polyoxyethylene sorbitan monolaurate); and (3) PFW, Tween 20, and sonication. Results suggest that vigorous recovery methods yield higher amounts of endotoxin from MWF samples than mild recovery methods in PFW alone. Additional studies are required to aid in the understanding of the factors that significantly affect endotoxin extraction yields from these fluids. [Description provided by NIOSH]
• Subjects:
  Biological Assay Endotoxins Microbiology Occupational Health Safety
• Keywords:
  Analytical Methods Analytical Processes Author Keywords: Endotoxin Bioassays Limulus Amoebocyte Lysate (LAL) Assay Metalworking Fluids Microorganisms
• ISSN:
  0002-8894
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DPSE - Division of Physical Sciences and Engineering
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  61
• Issue:
  4
• NIOSHTIC Number:
  nn:20020621
• Citation:
  Am Ind Hyg Assoc J 2000 Jul/Aug; 61(4):517-520
• Contact Point Address:
  U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Physical Sciences and Engineering, 4676 Columbia Parkway, Cincinnati, OH 4
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  True
• Source Full Name:
  American Industrial Hygiene Association Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9a23b5154e00c5f64b790106393a297671ddf04318c3b25a20510f0b6315071974b79846fdd78daf28afb94be1e631d243f9d1874a5776127a459422e1d609d5
• Download URL:
  https://stacks.cdc.gov/view/cdc/198318/cdc_198318_DS1.pdf
• File Type:
  [PDF - 322.00 KB ]