Involvement of 5′-Flanking kB-Like Sites Within Bcl-X Gene in Silica-Induced Bcl-X Expression

Details

• Personal Author:
  Castranova, Vincent ; Chen F ; Demers LM ; Shi X ; Vallyathan V ; Castranova, Vincent ; Chen F ; Demers LM ; Shi X ; Vallyathan V
• Description:
  The present study investigated the involvement of the transcription factor NF-kappaB in the expression of an anti-apoptotic gene, bcl-x, using a murine macrophage cell line and peritoneal macrophages from both wild type p50(+/+)) and NF-kappaB p50 gene knockout (p50(-/-)) mice. Increased expression of Bcl-x protein was observed in native and silica-exposed p50(-/-) macrophages in which the NF-kappaB-containing complex was predominantly induced. Co-transfection experiment using a bcl-x promoter reporter construct and an expression vector for NF-kappaB p50 or p65 indicates that p65, but not p50, up-regulates the promoter activity of the bcl-x gene. DNA sequence analysis revealed that there are several kappaB-like sites within the 5'-flanking region of the bcl-x gene. Electrophoretic mobility shift assay suggested differences in binding of the NF-kappaB complexes to these putative NF-kappaB binding sites of the bcl-x gene. [Description provided by NIOSH]
• Subjects:
  DNA Genetics Neoplasms Occupational Health Safety
• Keywords:
  Carcinomas Cellular-reactions DNA-damage In-vitro-studies
• ISSN:
  0021-9258
• Document Type:
  Text
• Genre:
  Journal Article ; Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  274
• Issue:
  50
• NIOSHTIC Number:
  nn:20000958
• Citation:
  J Biol Chem 1999 Dec; 274(50):35591-35595
• Contact Point Address:
  Pathology and Research Branch NIOSH 1095 Willowdale Rd. Morgantown, WV 26505
• Federal Fiscal Year:
  2000
• NORA Priority Area:
  Other Occupational Concerns ; Other Occupational Concerns
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Biological Chemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5057fcae34598eed2fa307a8a8079a313422e11046bb0c25493e3fa052a560aeda54b6b04b792d4feb63f9764f0f3beda5b4bccb43236fbebe4f5ea15aa314f8
• Download URL:
  https://stacks.cdc.gov/view/cdc/198272/cdc_198272_DS1.pdf
• File Type:
  [PDF - 177.60 KB ]